{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T23:37:51Z","timestamp":1725579471796},"reference-count":18,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The use of rapid tests for human immunodeficiency virus (HIV) has become standard in HIV testing algorithms employed in resource-limited settings. We report an extensive HIV rapid test validation study conducted among Ugandan blood bank donors at low risk for HIV infection. The operational characteristics of four readily available commercial HIV rapid test kits were first determined with 940 donor samples and were used to select a serial testing algorithm. Uni-Gold Recombigen HIV was used as the screening test, followed by HIV-1\/2 STAT-PAK for reactive samples. OraQuick HIV-1 testing was performed if the first two test results were discordant. This algorithm was then tested with 5,252 blood donor samples, and the results were compared to those of enzyme immunoassays (EIAs) and Western blotting. The unadjusted algorithm sensitivity and specificity were 98.6 and 99.9%, respectively. The adjusted sensitivity and specificity were 100 and 99.96%, respectively. This HIV testing algorithm is a suitable alternative to EIAs and Western blotting for Ugandan blood donors.<\/jats:p>","DOI":"10.1128\/jcm.00894-07","type":"journal-article","created":{"date-parts":[[2007,8,16]],"date-time":"2007-08-16T00:29:32Z","timestamp":1187224172000},"page":"3281-3285","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Large-Scale Human Immunodeficiency Virus Rapid Test Evaluation in a Low-Prevalence Ugandan Blood Bank Population"],"prefix":"10.1128","volume":"45","author":[{"given":"Leigh A.","family":"Eller","sequence":"first","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"},{"name":"U.S. Military Human Immunodeficiency Virus Research Program, Rockville, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Eller","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"},{"name":"U.S. Military Human Immunodeficiency Virus Research Program, Rockville, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benson J.","family":"Ouma","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Kataaha","sequence":"additional","affiliation":[{"name":"Uganda National Blood Transfusion Service, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard S.","family":"Bagaya","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Olemukan","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Erima","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian","family":"Kawala","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark S.","family":"de Souza","sequence":"additional","affiliation":[{"name":"U.S. Military Human Immunodeficiency Virus Research Program, Rockville, Maryland"},{"name":"Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Kibuuka","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred","family":"Wabwire-Mangen","sequence":"additional","affiliation":[{"name":"Makerere University Walter Reed Project, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila A.","family":"Peel","sequence":"additional","affiliation":[{"name":"Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"O'Connell","sequence":"additional","affiliation":[{"name":"Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merlin L.","family":"Robb","sequence":"additional","affiliation":[{"name":"Henry M. Jackson Foundation, Rockville, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson L.","family":"Michael","sequence":"additional","affiliation":[{"name":"Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.Supplement_2.S176"},{"key":"e_1_3_2_3_2","first-page":"321","volume":"73","year":"1998","unstructured":"Anonymous. 1998. The importance of simple\/rapid assays in HIV testing. Wkly. Epidemiol. Rec.73:321-326.","journal-title":"Wkly. Epidemiol. Rec."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1111\/j.1749-6632.2000.tb05475.x","volume":"918","year":"2000","unstructured":"Bakari, J. P., S. McKenna, A. Myrick, K. Mwinga, G. J. Bhat, and S. Allen. 2000. Rapid voluntary testing and counseling for HIV. Acceptability and feasibility in Zambian antenatal care clinics. Ann. N. Y. Acad. Sci.918:64-76.","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"e_1_3_2_5_2","first-page":"1158","volume":"279","year":"1998","unstructured":"Centers for Disease Control and Prevention. 1998. Update: HIV counseling and testing using rapid tests\u2014United States, 1995. JAMA279:1158-1159.","journal-title":"JAMA"},{"key":"e_1_3_2_6_2","first-page":"538","volume":"50","year":"2001","unstructured":"Centers for Disease Control and Prevention. 2001. Routinely recommended HIV testing at an urban urgent-care clinic\u2014Atlanta, Georgia, 2000. Morb. Mortal. Wkly. Rep.50:538-541.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/26.4.404"},{"key":"e_1_3_2_8_2","unstructured":"Clinical epidemiology: the essentials 1988"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3850-3852.2004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39210.582801.BE"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10461-006-9073-y"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1994.03510330081039"},{"key":"e_1_3_2_13_2","first-page":"81","volume":"72","year":"1997","unstructured":"Joint United Nations Programme on HIV\/AIDS-World Health Organization. 1997. Revised recommendations for the selection and use of HIV antibody tests. Wkly. Epidemiol. Rec.72:81-87.","journal-title":"Wkly. Epidemiol. Rec."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.5.1808-1812.2001"},{"key":"e_1_3_2_15_2","first-page":"S103","volume":"11","year":"1997","unstructured":"McKenna, S. L., G. K. Muyinda, D. Roth, M. Mwali, N. Ng'andu, A. Myrick, C. Luo, F. H. Priddy, V. M. Hall, A. A. von Lieven, J. R. Sabatino, K. Mark, and S. A. Allen. 1997. Rapid HIV testing and counseling for voluntary testing centers in Africa. AIDS11(Suppl. 1):S103-S110.","journal-title":"AIDS"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2005.01.023"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(03)00169-1"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.5312-5315.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3868-3869.2003"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00894-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00894-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T12:02:25Z","timestamp":1708171345000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00894-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1128\/JCM.00894-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00894-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]},"assertion":[{"value":"2007-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}