{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T22:04:23Z","timestamp":1769205863426,"version":"3.49.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            is a leading pathogen of epidemic bacterial meningitis and fulminant sepsis worldwide. Twelve different\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            serogroups have been identified to date based on antigenic differences in the capsular polysaccharide. However, more than 90% of human cases of\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            meningitis are the result of infection with just five serogroups, A, B, C, W135, and Y. Efficient methods of detection and genogrouping of\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            isolates are needed, therefore, in order to monitor prevalent serogroups as a means of disease control and prevention. The capsular gene complex regions have been sequenced from only seven out of the 12 serogroups. In this study, the capsular gene complexes of the remaining five serogroups were sequenced and analyzed. Primers were designed that were specific for\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            species and for the 12 individual serogroups, and a multiplex PCR assay using these specific primers was developed for\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            detection and genogrouping. The assay was tested using 15 reference strains covering all 12 serogroups, 143 clinical isolates, and 21 strains from closely related species or from species that cause meningitis. The assay could detect\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            serogroups and was shown to be specific, with a detection sensitivity of 1 ng of genomic DNA (equivalent to \u223c4 \u00d7 10\n            <jats:sup>5<\/jats:sup>\n            genomes) or 3 \u00d7 10\n            <jats:sup>5<\/jats:sup>\n            CFU\/ml in noncultured mock cerebrospinal fluid (CSF) specimens. This study, therefore, describes for the first time the development of a molecular protocol for the detection of all\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            serogroups. This multiplex PCR-based assay may have use for the clinical diagnosis and epidemiological surveillance of\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.00918-11","type":"journal-article","created":{"date-parts":[[2011,11,17]],"date-time":"2011-11-17T12:38:48Z","timestamp":1321533528000},"page":"46-51","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Development of a Multiplex PCR Assay for Detection and Genogrouping of Neisseria meningitidis"],"prefix":"10.1128","volume":"50","author":[{"given":"Hongfei","family":"Zhu","sequence":"first","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"},{"name":"The Engineering and Research Center for Microbial Functional Genomics and Detection Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liuqing","family":"Wen","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"The Engineering and Research Center for Microbial Functional Genomics and Detection Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Xu","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhujun","family":"Shao","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingliang","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter R.","family":"Reeves","sequence":"additional","affiliation":[{"name":"School of Molecular and Microbial Biosciences (G08), University of Sydney, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boyang","family":"Cao","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China"},{"name":"Tianjin Research Center for Functional Genomics and Biochip, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.276"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.1127-1131.2006"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12862.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06580.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/PL00008618","article-title":"Molecular divergence of the sia locus in different serogroups of Neisseria meningitidis expressing polysialic acid capsules","volume":"257","author":"Claus H","year":"1997","unstructured":"ClausH VogelU MuhlenhoffM Gerardy-SchahnR FroschM. 1997. 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