{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:36:30Z","timestamp":1788989790988,"version":"build-2803163510"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Invasive mold infections are life-threatening diseases for which appropriate antifungal therapy is crucial. Their epidemiology is evolving, with the emergence of triazole-resistant\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            spp. and multidrug-resistant non-\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            molds. Despite the lack of interpretive criteria, antifungal susceptibility testing of molds may be useful in guiding antifungal therapy. The standard broth microdilution method (BMD) is demanding and requires expertise. We assessed the performance of a commercialized gradient diffusion method (Etest method) as an alternative to BMD. The MICs or minimal effective concentrations (MECs) of amphotericin B, voriconazole, posaconazole, caspofungin, and micafungin were assessed for 290 clinical isolates of the most representative pathogenic molds (154\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            and 136 non-\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            isolates) with the BMD and Etest methods. Essential agreements (EAs) within \u00b12 dilutions of \u226590% between the two methods were considered acceptable. EAs for amphotericin B and voriconazole were &gt;90% for most potentially susceptible species. For posaconazole, the correlation was acceptable for\n            <jats:named-content content-type=\"genus-species\">Mucoromycotina<\/jats:named-content>\n            but Etest MIC values were consistently lower for\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            spp. (EAs of &lt;90%). Excellent EAs were found for echinocandins with highly susceptible (MECs of &lt;0.015 \u03bcg\/ml) or intrinsically resistant (MECs of &gt;16 \u03bcg\/ml) strains. However, MEC determinations lacked consistency between methods for strains exhibiting mid-range MECs for echinocandins. We concluded that the Etest method is an appropriate alternative to BMD for antifungal susceptibility testing of molds under specific circumstances, including testing with amphotericin B or triazoles for non-\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            molds (\n            <jats:named-content content-type=\"genus-species\">Mucoromycotina<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Fusarium<\/jats:named-content>\n            spp.). Additional study of molecularly characterized triazole-resistant\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            isolates is required to confirm the ability of the Etest method to detect voriconazole and posaconazole resistance among\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            spp.\n          <\/jats:p>","DOI":"10.1128\/jcm.00925-15","type":"journal-article","created":{"date-parts":[[2015,7,23]],"date-time":"2015-07-23T01:40:23Z","timestamp":1437615623000},"page":"3176-3181","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Comparing Etest and Broth Microdilution for Antifungal Susceptibility Testing of the Most-Relevant Pathogenic Molds"],"prefix":"10.1128","volume":"53","author":[{"given":"Fr\u00e9d\u00e9ric","family":"Lamoth","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and International Health, Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"Clinical Microbiology Laboratory, Department of Pathology, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"Infectious Diseases Service, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland"},{"name":"Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara D.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"Clinical Microbiology Laboratory, Department of Pathology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/651263"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/651262"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02136-10"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.01978.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks137"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06133-11"},{"key":"e_1_3_3_9_2","volume-title":"M38-A2","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard\u20142nd ed. M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"2"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00862-09"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780801975576"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1360-1367.2001"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.403-409.2003"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01187-06"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.2101-2107.2002"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01677-09"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg384"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1126-1129.2003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(02)00544-8"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3359-3361.2000"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5270-5272.2003"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1480-1483.1999"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2339-2341.2003"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05910-11"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05763.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02487-12"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00925-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00925-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:00:42Z","timestamp":1646481642000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00925-15"}},"subtitle":[],"editor":[{"given":"D. J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,10]]},"references-count":25,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1128\/JCM.00925-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00925-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10]]},"assertion":[{"value":"2015-04-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}