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Multidrug-resistant and epidemic strains are a large proportion of HAI agents, and multidrug-resistant strains of\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            , a leading HAI agent, have caused an urgent public health crisis. In the health care environment, patient colonization by\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            precedes infection, and transmission via colonization leads to outbreaks. Periodic patient screening for\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            colonization has the potential to curb the number of HAIs. In this report, we describe the design and validation of KlebSeq, a highly informative screening tool that detects\n            <jats:named-content content-type=\"genus-species\">Klebsiella<\/jats:named-content>\n            species and identifies clinically important strains and characteristics by using highly multiplexed amplicon sequencing without a live-culturing step. We demonstrate the utility of this tool on several complex specimen types, including urine, wound swabs and tissue, and several types of respiratory and fecal specimens, showing\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            species and clonal group identification and antimicrobial resistance and virulence profiling, including capsule typing. Use of this amplicon sequencing tool to screen patients for\n            <jats:named-content content-type=\"genus-species\">Klebsiella<\/jats:named-content>\n            carriage could inform health care staff of the risk of infection and outbreak potential. KlebSeq also serves as a model for next-generation molecular tools for public health and health care, as expansion of this tool can be used for several other HAI agents or applications.\n          <\/jats:p>","DOI":"10.1128\/jcm.00927-16","type":"journal-article","created":{"date-parts":[[2016,8,11]],"date-time":"2016-08-11T02:49:08Z","timestamp":1470883748000},"page":"2582-2596","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae"],"prefix":"10.1128","volume":"54","author":[{"given":"Jolene R.","family":"Bowers","sequence":"first","affiliation":[{"name":"Translational Genomics Research Institute, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darrin","family":"Lemmer","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason W.","family":"Sahl","sequence":"additional","affiliation":[{"name":"Microbial Genetics and Genomics, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talima","family":"Pearson","sequence":"additional","affiliation":[{"name":"Microbial Genetics and Genomics, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M.","family":"Driebe","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bette","family":"Wojack","sequence":"additional","affiliation":[{"name":"Banner Health, Phoenix, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Saubolle","sequence":"additional","affiliation":[{"name":"Banner Health, Phoenix, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Engelthaler","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Keim","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute, Flagstaff, Arizona, USA"},{"name":"Microbial Genetics and Genomics, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/1.5.736"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1306801"},{"key":"e_1_3_3_4_2","volume-title":"Antibiotic resistance threats in the United States, 2013","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. 2013. 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