{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:58:37Z","timestamp":1791406717387,"version":"4.3.3"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Rift Valley fever (RVF) virus is a mosquito-borne virus associated with large-scale epizootics\/epidemics throughout Africa and the Arabian peninsula. Virus infection can result in economically disastrous \u201cabortion storms\u201d and high newborn mortality in livestock. Human infections result in a flu-like illness, with 1 to 2% of patients developing severe complications, including encephalitis or hemorrhagic fever with high fatality rates. There is a critical need for a highly sensitive and specific molecular diagnostic assay capable of detecting the natural genetic spectrum of RVF viruses. We report here the establishment of a pan-RVF virus quantitative real-time reverse transcription-PCR assay with high analytical sensitivity (\u223c5 RNA copies of in vitro-transcribed RNA\/reaction or \u223c0.1 PFU of infectious virus\/reaction) and efficiency (standard curve slope = \u22123.66). Based on the alignments of the complete genome sequences of 40 ecologically and biologically diverse virus isolates collected over 56 years (1944 to 2000), the primer and probe annealing sites targeted in this assay are known to be located in highly conserved genomic regions. The performance of this assay relative to serologic assays is illustrated by testing of known RVF case materials obtained during the Saudi Arabia outbreak in 2000. Furthermore, analysis of acute-phase blood samples collected from human patients (25 nonfatal, 8 fatal) during that outbreak revealed that patient viremia at time of presentation at hospital may be a useful prognostic tool in determining patient outcome.<\/jats:p>","DOI":"10.1128\/jcm.00936-07","type":"journal-article","created":{"date-parts":[[2007,9,6]],"date-time":"2007-09-06T01:27:29Z","timestamp":1189042049000},"page":"3506-3513","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":162,"title":["Highly Sensitive and Broadly Reactive Quantitative Reverse Transcription-PCR Assay for High-Throughput Detection of Rift Valley Fever Virus"],"prefix":"10.1128","volume":"45","author":[{"given":"Brian H.","family":"Bird","sequence":"first","affiliation":[{"name":"Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G-14, Atlanta, Georgia 30329"},{"name":"University of California, Davis, School of Veterinary Medicine, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darcy A.","family":"Bawiec","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G-14, Atlanta, Georgia 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas G.","family":"Ksiazek","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G-14, Atlanta, Georgia 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor R.","family":"Shoemaker","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G-14, Atlanta, Georgia 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart T.","family":"Nichol","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G-14, Atlanta, Georgia 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"982","volume":"49","year":"2000","unstructured":"Anonymous. 2000. Update: outbreak of Rift Valley Fever-Saudi Arabia, August-November 2000. Morb. Mortal. Wkly. Rep.49:982-985.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02095-06"},{"key":"e_1_3_2_4_2","first-page":"205","volume":"44","year":"1977","unstructured":"Coetzer, J. A. 1977. The pathology of Rift Valley fever. I. Lesions occurring in natural cases in new-born lambs. Onderstepoort J. Vet. Res.44:205-211.","journal-title":"Onderstepoort J. Vet. Res."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2323-2330.2002"},{"key":"e_1_3_2_6_2","first-page":"470","volume":"23","year":"1962","unstructured":"Easterday, B. C., M. H. McGavran, J. R. Rooney, and L. C. Murphy. 1962. The pathogenesis of Rift Valley fever in lambs. Am. J. Vet. Res.23:470-479.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)99624-7"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4456-4461.2001"},{"key":"e_1_3_2_9_2","unstructured":"Diseases of sheep. Kenya Veterinary Department annual report 1910 1910"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5426.397"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/378747"},{"key":"e_1_3_2_12_2","first-page":"803","volume":"58","year":"1980","unstructured":"McIntosh, B. M., D. Russell, I. dos Santos, and J. H. Gear. 1980. Rift Valley fever in humans in South Africa. S. Afr. Med. J.58:803-806.","journal-title":"S. Afr. Med. J."},{"key":"e_1_3_2_13_2","first-page":"114","volume":"3","year":"1981","unstructured":"Meegan, J. M. 1981. Rift Valley fever in Egypt: an overview of the epizootics in 1977 and 1978. Contrib. Epidemiol. Biostat.3:114-123.","journal-title":"Contrib. Epidemiol. Biostat."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2516(06)80134-2"},{"key":"e_1_3_2_15_2","first-page":"1581","year":"2001","unstructured":"Schmaljohn, C. S., and J. W. Hooper. 2001. Bunyaviridae: the viruses and their replication, p. 1581-1602. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 4th ed. Lippincott-Raven Publishers, Philadelphia, PA.","journal-title":"Fields virology"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0812.020195"},{"key":"e_1_3_2_17_2","unstructured":"J. Infect. Dis."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00936-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00936-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:35:49Z","timestamp":1646458549000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00936-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2007,11]]}},"alternative-id":["10.1128\/JCM.00936-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00936-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,11]]},"assertion":[{"value":"2007-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}