{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:18:51Z","timestamp":1774437531602,"version":"3.50.1"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2020,7,23]]},"abstract":"<jats:p>\n                    We compared the ability of 2 commercial molecular amplification assays (RealTime SARS-CoV-2 on the\n                    <jats:italic>m<\/jats:italic>\n                    2000 [abbreviated ACOV; Abbott] and ID Now COVID-19 [abbreviated IDNOW; Abbott]) and a laboratory-developed test (modified CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extraction by eMag [bioM\u00e9rieux] and amplification on QuantStudio 6 or ABI 7500 real-time PCR system [abbreviated CDC COV]) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in upper respiratory tract specimens.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00938-20","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T08:49:00Z","timestamp":1590655740000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2"],"prefix":"10.1128","volume":"58","author":[{"given":"Nicholas M.","family":"Moore","sequence":"first","affiliation":[{"name":"Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA"},{"name":"Department of Internal Medicine, Division of Infectious Diseases, Rush University Medical Center, Chicago, Illinois, USA"},{"name":"Department of Medical Laboratory Science, Rush University, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debra","family":"Schejbal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Lindsley","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary K.","family":"Hayden","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA"},{"name":"Department of Internal Medicine, Division of Infectious Diseases, Rush University Medical Center, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2008-3"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.3204"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.6.2000094"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.9.2000178"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30231-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30370-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001191"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2020.35.e61"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30607-3"},{"key":"e_1_3_2_12_2","volume-title":"Abbott RealTime SARS-CoV-2 assay package insert","author":"Abbott Laboratories","unstructured":"Abbott Laboratories. Abbott RealTime SARS-CoV-2 assay package insert. Abbott Laboratories, Chicago, IL. https:\/\/www.molecular.abbott\/sal\/9N77-095_SARS-CoV-2_US_EUA_Amp_PI.pdf."},{"key":"e_1_3_2_13_2","volume-title":"CDC 2019-novel coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel services","author":"Centers for Disease Control and Prevention","year":"2020","unstructured":"Centers for Disease Control and Prevention. 2020. CDC 2019-novel coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel services. Centers for Disease Control and Prevention, Atlanta, GA. https:\/\/www.fda.gov\/media\/134922\/download."},{"key":"e_1_3_2_14_2","volume-title":"ID Now COVID-19 package insert","author":"Abbott Laboratories","unstructured":"Abbott Laboratories. ID Now COVID-19 package insert. Abbott Laboratories, Chicago, IL. https:\/\/www.fda.gov\/media\/136525\/download."},{"key":"e_1_3_2_15_2","unstructured":"Food and Drug Administration. Policy for diagnostics tests for coronavirus disease-2019 during the public health emergency. Food and Drug Administration Washington DC. https:\/\/www.fda.gov\/media\/135659\/download. Accessed 17 March 2020."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00798-20"},{"key":"e_1_3_2_17_2","volume-title":"User protocol for evaluation of qualitative test performance; approved guideline","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. User protocol for evaluation of qualitative test performance; approved guideline\u20142nd ed. CLSI document EP12-A2. 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