{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:56:49Z","timestamp":1787061409346,"version":"build-2736575974"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biochemical methods employed to classify bacterial species have limitations and may have contributed to the taxonomic complexity recently reported for the genus\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    . The objective of the present study was to apply a simple biochemical test panel to classify a collection of human\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    isolates. We found that with only three additional tests, it is possible to place most isolates in a defined species. Analysis of a 512-bp sequence of the\n                    <jats:italic>rpoB<\/jats:italic>\n                    gene was used as the reference. A total of 16 conventional and 4 supplementary tests were used to evaluate 122 recent isolates identified as\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    from 120 patients, isolated at the clinical laboratory of a university hospital in Minas Gerais, Brazil. Of these, 102 (84%) isolates were identified as\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    or\n                    <jats:italic>Klebsiella variicola<\/jats:italic>\n                    , 19 (15%) as\n                    <jats:italic>Klebsiella oxytoca<\/jats:italic>\n                    , and 1 (1%) as\n                    <jats:italic>Raoultella planticola<\/jats:italic>\n                    . Enterobacterial repetitive intergenic consensus-PCR typing revealed a diversity of genotypes.\n                    <jats:italic>rpoB<\/jats:italic>\n                    gene sequencing confirmed the phenotypic identification and detected five\n                    <jats:italic>K. variicola<\/jats:italic>\n                    isolates among the\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    \/\n                    <jats:italic>K. variicola<\/jats:italic>\n                    group. Three additional tests that include growth at 10\u00b0C and histamine and\n                    <jats:sc>d<\/jats:sc>\n                    -melezitose assimilation should be considered essential tests for the typing of\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    isolates.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00940-06","type":"journal-article","created":{"date-parts":[[2006,10,4]],"date-time":"2006-10-04T13:59:53Z","timestamp":1159970393000},"page":"3640-3646","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":85,"title":["Identification of Clinical Isolates of Indole-Positive and Indole-Negative\n                    <i>Klebsiella<\/i>\n                    spp"],"prefix":"10.1128","volume":"44","author":[{"given":"Maria Silvana","family":"Alves","sequence":"first","affiliation":[{"name":"Faculdade de Farma\u0301cia e Bioqui\u0301mica, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais"},{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubens Clayton da Silva","family":"Dias","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela Christina Dias","family":"de Castro","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee W.","family":"Riley","sequence":"additional","affiliation":[{"name":"School of Public Health, University of California, Berkeley, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Meurer","family":"Moreira","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)80014-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.8.2230-2230.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.05.003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00228"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.11.010"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-3-915"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.00973.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.11.2915-2917.1997"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-4-1695"},{"key":"e_1_3_2_11_2","unstructured":"Clinical and Laboratory Standards Institute. 2005. 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