{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T23:13:31Z","timestamp":1771456411331,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To improve the rate of detection of\n            <jats:named-content content-type=\"genus-species\">Clonorchis sinensis<\/jats:named-content>\n            infection, we compared different specimens from patients with cholecystolithiasis. Feces, gallbladder bile, and gallbladder stones collected from 179 consecutive patients with cholecystolithiasis underwent microscopic examination, and according to the results, 30 egg-positive and 30 egg-negative fecal, gallbladder bile, and gallbladder stone specimens, respectively, underwent real-time fluorescent PCR. The detection rates of eggs in feces, bile, and gallbladder stones were 30.7%, 44.7%, and 69.8%, respectively, and the differences were statistically significant (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01). The PCR results confirmed that the eggs in the specimens were\n            <jats:named-content content-type=\"genus-species\">C. sinensis<\/jats:named-content>\n            eggs. Eggs in the feces were \u201cfresh\u201d and in the gallbladder stones were \u201cold.\u201d Microscopic examination of gallbladder stones may improve the detection rates of\n            <jats:named-content content-type=\"genus-species\">C. sinensis<\/jats:named-content>\n            infection, which is important for developing individualized treatments to prevent the recurrence of gallbladder stones and to prevent the occurrence of severe liver damage and cholangiocarcinoma.\n          <\/jats:p>","DOI":"10.1128\/jcm.00946-13","type":"journal-article","created":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T12:25:07Z","timestamp":1369311907000},"page":"2551-2555","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Microscopic Examination of Gallbladder Stones Improves Rate of Detection of Clonorchis sinensis Infection"],"prefix":"10.1128","volume":"51","author":[{"given":"Tie","family":"Qiao","sequence":"first","affiliation":[{"name":"Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China"},{"name":"The Second People's Hospital of Panyu, Nansha, Guangzhou, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui-hong","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China"},{"name":"The Second People's Hospital of Panyu, Nansha, Guangzhou, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-bing","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China"},{"name":"The Second People's Hospital of Panyu, Nansha, Guangzhou, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-ming","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China"},{"name":"The Second People's Hospital of Panyu, Nansha, Guangzhou, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen-liang","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China"},{"name":"The Second People's Hospital of Panyu, Nansha, Guangzhou, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liu-qing","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China"},{"name":"The Second People's Hospital of Panyu, Nansha, Guangzhou, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2005300"},{"key":"e_1_3_2_3_2","first-page":"581","volume-title":"International handbook of foodborne pathogens","author":"Hong ST","year":"2003","unstructured":"HongST. 2003. 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