{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:33:34Z","timestamp":1762101214817},"reference-count":18,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have developed a new research assay that combines sequence-specific sample preparation and isothermal amplification for the detection of\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            infections. The assay targets both the\n            <jats:italic>omp<\/jats:italic>\n            gene and the cryptic plasmid of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and the multicopy\n            <jats:italic>opa<\/jats:italic>\n            gene of\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            , which are amplified and detected in a single reaction. We evaluated the ability of the assay to detect\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            infections in first-catch urine, swab, and liquid-based cytology samples. Total agreement between the new assay and APTIMA Combo 2 varied between 95.3% and 100%, depending on the sample type and target detected. Total agreement between the new assay and BD ProbeTec varied between 96.7% and 100%, depending on the sample type and target detected. The assay has a simple work flow, and endpoint results can be achieved in 3 h, including sample preparation. The assay described here was evaluated for research use and was compared to commercially available assays.\n          <\/jats:p>","DOI":"10.1128\/jcm.00952-11","type":"journal-article","created":{"date-parts":[[2011,9,29]],"date-time":"2011-09-29T02:08:15Z","timestamp":1317262095000},"page":"4121-4125","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of Chlamydia trachomatis and Neisseria gonorrhoeae Detection in Urine, Endocervical, and Vaginal Specimens by a Multiplexed Isothermal Thermophilic Helicase-Dependent Amplification (tHDA) Assay"],"prefix":"10.1128","volume":"49","author":[{"given":"Dominic","family":"O'Neil","sequence":"first","affiliation":[{"name":"Qiagen, Inc., 1201 Clopper Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Doseeva","sequence":"additional","affiliation":[{"name":"Qiagen, Inc., 1201 Clopper Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Rothmann","sequence":"additional","affiliation":[{"name":"Qiagen, Inc., 1201 Clopper Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Wolff","sequence":"additional","affiliation":[{"name":"Qiagen, Inc., 1201 Clopper Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Nazarenko","sequence":"additional","affiliation":[{"name":"Qiagen, Inc., 1201 Clopper Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503096200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006985"},{"key":"e_1_3_2_4_2","volume-title":"Sexually transmitted disease surveillance, 2009","author":"Centers for Disease Control and Prevention","year":"2010","unstructured":"Centers for Disease Control and Prevention. 2010. Sexually transmitted disease surveillance, 2009. 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