{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:32:28Z","timestamp":1787016748443,"version":"build-2736575974"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A syphilis diagnosis is often aided by the detection of treponemal and nontreponemal antibodies. Automated treponemal antibody detection systems enable high-volume clinical laboratories to perform syphilis screening at a faster pace with lower labor costs. The Lumipulse G TP-N chemiluminescent immunoassay is an automated system that qualitatively detects IgG and IgM antibodies against\n                    <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n                    antigens in human serum and plasma. To assess performance characteristics and workflow efficiency, the Lumipulse G TP-N assay was compared to the Bioplex 2200 Syphilis IgG multiplex flow immunoassay. Among the 4,134 routine and HIV samples tested by the two automated assays, the percentage of agreement was excellent at 99.0% (95% confidence interval [CI], 98.6% to 99.2%; \u03ba, 0.89), with the Lumipulse G TP-N having a shorter time to first and subsequent results. All specimens with reactive syphilis screening results were further tested by rapid plasma reagin (RPR) and\n                    <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n                    particle agglutination (TP\u00b7PA) testing (\n                    <jats:italic>n<\/jats:italic>\n                    = 231). The results from the RPR-reactive samples (\n                    <jats:italic>n<\/jats:italic>\n                    = 82) showed complete concordance with the two automated assays, while the TP\u00b7PA assay displayed some discrepancies. The positive percent agreement (PPA) and negative percent agreement (NPA) between the TP\u00b7PA test and the Lumipulse G TP-N test were 98.9% and 77.3%, respectively. The Bioplex 2200 Syphilis IgG immunoassay displayed a similar PPA (100%) but a substantially lower NPA (15.9%). Patient chart reviews of discrepant results suggested that the Lumipulse G TP-N assay produced 27 fewer falsely reactive results and can reduce the amount of additional confirmatory RPR and TP\u00b7PA testing needed. The analogous performance characteristics of the two automated systems indicate that the Lumipulse G TP-N assay is suitable for high-throughput syphilis screening.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00966-17","type":"journal-article","created":{"date-parts":[[2017,9,6]],"date-time":"2017-09-06T23:16:29Z","timestamp":1504739789000},"page":"3236-3241","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the Lumipulse G TP-N Chemiluminescent Immunoassay as a Syphilis Screening Test"],"prefix":"10.1128","volume":"55","author":[{"given":"Daniel A.","family":"Ortiz","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Loeffelholz","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(16)32411-4"},{"key":"e_1_3_2_3_2","volume-title":"Sexually transmitted disease surveillance 2015","author":"CDC","year":"2016","unstructured":"CDC. 2016. Sexually transmitted disease surveillance 2015. U.S. Department of Health and Human Services, Atlanta, GA."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181ca3c0a"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/PHH.0b013e3182113954"},{"key":"e_1_3_2_6_2","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2015","volume":"64","author":"Workowski KA","year":"2015","unstructured":"Workowski KA Bolan GA ; Centers for Disease Control and Prevention. 2015. Sexually transmitted diseases treatment guidelines, 2015. MMWR Recomm Rep64(RR-03):1\u2013137.","journal-title":"MMWR Recomm Rep"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e318186debb"},{"key":"e_1_3_2_8_2","first-page":"872","article-title":"Syphilis testing algorithms using treponemal tests for initial screening\u2014four laboratories, New York City, 2005\u20132006","volume":"57","author":"CDC","year":"2008","unstructured":"CDC. 2008. Syphilis testing algorithms using treponemal tests for initial screening\u2014four laboratories, New York City, 2005\u20132006. MMWR Morb Mortal Wkly Rep57:872\u2013875.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06347-11"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00086-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00316-13"},{"key":"e_1_3_2_12_2","unstructured":"Egglestone\nSI\nTurner\nAJ\n. 2000. Serological diagnosis of syphilis. PHLS Syphilis Serology Working Group. Commun Dis Public Health3:158\u2013162."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2008.008510"},{"key":"e_1_3_2_14_2","first-page":"133","article-title":"Discordant results from reverse sequence syphilis screening\u2014five laboratories, United States, 2006\u20132010","volume":"60","author":"CDC","year":"2011","unstructured":"CDC. 2011. Discordant results from reverse sequence syphilis screening\u2014five laboratories, United States, 2006\u20132010. MMWR Morb Mortal Wkly Rep60:133\u2013137.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05636-11"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181f07e91"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31824e53f7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.21839"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00116-15"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2015.150625"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00966-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00966-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T03:50:14Z","timestamp":1646452214000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00966-17"}},"subtitle":[],"editor":[{"given":"Erik","family":"Munson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":19,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1128\/JCM.00966-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00966-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11]]},"assertion":[{"value":"2017-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}