{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T06:56:24Z","timestamp":1783666584231,"version":"3.55.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Accurate point-of-care (POC) diagnostic tests for\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            infection are urgently needed for the rapid treatment of patients. In a blind comparative study, we evaluated microwave-accelerated metal-enhanced fluorescence (MAMEF) assays for ultrafast and sensitive detection of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            DNA from vaginal swabs. The results of two distinct MAMEF assays were compared to those of nucleic acid amplification tests (NAATs). The first assay targeted the\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            16S rRNA gene, and the second assay targeted the\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            cryptic plasmid. Using pure\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            , the MAMEF assays detected as few as 10 inclusion-forming units\/ml of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            in less than 9 min, including DNA extraction and detection. A total of 257 dry vaginal swabs from 245 female adolescents aged 14 to 22 years were analyzed. Swabs were eluted with water, the solutions were lysed to release and to fragment genomic DNA, and MAMEF-based DNA detection was performed. The prevalence of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            by NAATs was 17.5%. Of the 45 samples that were\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            positive and the 212 samples that were\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            negative by NAATs, 33\/45 and 197\/212 were correctly identified by the MAMEF assays if both assays were required to be positive (sensitivity, 73.3%; specificity, 92.9%). Using the plasmid-based assay alone, 37\/45\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            -positive and 197\/212\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            -negative samples were detected (sensitivity, 82.2%; specificity, 92.9%). Using the 16S rRNA assay alone, 34\/45\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            -positive and 197\/212\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            -negative samples were detected (sensitivity, 75.5%; specificity, 92.9%). The overall rates of agreement with NAAT results for the individual 16S rRNA and cryptic plasmid assays were 89.5% and 91.0%, respectively. Given the sensitivity, specificity, and rapid detection of the plasmid-based assay, the plasmid-based MAMEF assay appears to be suited for clinical POC testing.\n          <\/jats:p>","DOI":"10.1128\/jcm.00980-13","type":"journal-article","created":{"date-parts":[[2013,6,27]],"date-time":"2013-06-27T04:23:55Z","timestamp":1372307035000},"page":"2913-2920","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Blind Evaluation of the Microwave-Accelerated Metal-Enhanced Fluorescence Ultrarapid and Sensitive Chlamydia trachomatis Test by Use of Clinical Samples"],"prefix":"10.1128","volume":"51","author":[{"given":"Johan H.","family":"Melendez","sequence":"first","affiliation":[{"name":"Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill S.","family":"Huppert","sequence":"additional","affiliation":[{"name":"Cincinnati Children's Hospital Medical Center, Division of Gynecology, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Jett-Goheen","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University Medical School, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Hesse","sequence":"additional","affiliation":[{"name":"Cincinnati Children's Hospital Medical Center, Division of Gynecology, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Quinn","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University Medical School, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte A.","family":"Gaydos","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University Medical School, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris D.","family":"Geddes","sequence":"additional","affiliation":[{"name":"Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Sexually transmitted disease surveillance, 2011","author":"Centers for Disease Control and Prevention","year":"2012","unstructured":"Centers for Disease Control and Prevention. 2012. 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