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Analysis of 100<jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>isolates, 28<jats:named-content content-type=\"genus-species\">Haemophilus haemolyticus<\/jats:named-content>isolates, and 14 other bacterial species revealed 100% sensitivity (95% confidence interval [CI], 96% to 100%) and 100% specificity (95% CI, 92% to 100%) for this assay. The evaluation of 80 clinical specimens demonstrated a strong correlation between semiquantitative culture and the qPCR (<jats:italic>P<\/jats:italic>&lt; 0.001).<\/jats:p>","DOI":"10.1128\/jcm.00982-16","type":"journal-article","created":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T05:34:34Z","timestamp":1466746474000},"page":"2380-2383","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains"],"prefix":"10.1128","volume":"54","author":[{"given":"Camilla","family":"de Gier","sequence":"first","affiliation":[{"name":"School of Paediatrics and Child Health, University of Western Australia, Perth, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janessa L.","family":"Pickering","sequence":"additional","affiliation":[{"name":"School of Paediatrics and Child Health, University of Western Australia, Perth, Australia"},{"name":"Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Richmond","sequence":"additional","affiliation":[{"name":"School of Paediatrics and Child Health, University of Western Australia, Perth, Australia"},{"name":"Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia"},{"name":"Department of Paediatrics, Princess Margaret Hospital for Children, Subiaco, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth B.","family":"Thornton","sequence":"additional","affiliation":[{"name":"School of Paediatrics and Child Health, University of Western Australia, Perth, Australia"},{"name":"Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea-Ann S.","family":"Kirkham","sequence":"additional","affiliation":[{"name":"School of Paediatrics and Child Health, University of Western Australia, Perth, Australia"},{"name":"Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05476-11"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46346-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2010.1651"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0800353"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1097\/01.inf.0000148233.57296.90","article-title":"Haemophilus influenzae: a significant pathogen in acute otitis media","volume":"23","author":"Leibovitz E","year":"2004","unstructured":"Leibovitz E, Jacobs MR, Dagan R. 2004. 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