{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T09:19:12Z","timestamp":1742807952295},"reference-count":22,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T00:00:00Z","timestamp":1479859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Currently, conventional enzyme immunoassays which use manual gold immunoassays and colloidal tests (GICTs) are used as screening tools to detect\n            <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n            (syphilis), hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus type 1 (HIV-1), and HIV-2 in patients undergoing surgery. The present observational, cross-sectional study compared the sensitivity, specificity, and work flow characteristics of the conventional algorithm with manual GICTs with those of a newly proposed algorithm that uses the automated Bio-Flash technology as a screening tool in patients undergoing gastrointestinal (GI) endoscopy. A total of 956 patients were examined for the presence of serological markers of infection with HIV-1\/2, HCV, HBV, and\n            <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n            . The proposed algorithm with the Bio-Flash technology was superior for the detection of all markers (100.0% sensitivity and specificity for detection of anti-HIV and anti-HCV antibodies, HBV surface antigen [HBsAg], and\n            <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n            ) compared with the conventional algorithm based on the manual method (80.0% sensitivity and 98.6% specificity for the detection of anti-HIV, 75.0% sensitivity for the detection of anti-HCV, 94.7% sensitivity for the detection of HBsAg, and 100% specificity for the detection of anti-HCV and HBsAg) in these patients. The automated Bio-Flash technology-based screening algorithm also reduced the operation time by 85.0% (205 min) per day, saving up to 24 h\/week. In conclusion, the use of the newly proposed screening algorithm based on the automated Bio-Flash technology can provide an advantage over the use of conventional algorithms based on manual methods for screening for HIV, HBV, HCV, and syphilis before GI endoscopy.\n          <\/jats:p>","DOI":"10.1128\/jcm.00986-16","type":"journal-article","created":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T02:59:05Z","timestamp":1475722745000},"page":"3000-3006","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Screening for Human Immunodeficiency Virus, Hepatitis B Virus, Hepatitis C Virus, and Treponema pallidum by Blood Testing Using a Bio-Flash Technology-Based Algorithm before Gastrointestinal Endoscopy"],"prefix":"10.1128","volume":"54","author":[{"given":"Zhou","family":"Jun","sequence":"first","affiliation":[{"name":"Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Zhen","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhang","family":"QuiuLi","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An","family":"YuanQi","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica Vocero","family":"Casado","sequence":"additional","affiliation":[{"name":"Marketing Department, Biokit, Lli\u00e7\u00e0 d\u2032Amunt, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2008.01.027"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-012-0373-z"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.115623"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2009.11.012"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-003-0045-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i25.3953"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(10)60055-7"},{"key":"e_1_3_2_9_2","volume-title":"Screening donated blood for transfusion-transmissible infections: recommendations","author":"World Health Organization","year":"2009","unstructured":"World Health Organization. 2009. 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