{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T01:34:17Z","timestamp":1773884057559,"version":"3.50.1"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In June 2010, a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            -negative, ertapenem-resistant ST-258\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            strain was isolated from a patient in the Laniado Medical Center (LMC). Our aims were (i) to describe its molecular characteristics and resistance mechanisms and (ii) to assess whether the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            -negative ST-258\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            clone spreads as efficiently as its KPC-producing isogenic strain. In a prospective study, surveillance of all ertapenem-resistant, carbapenemase-negative\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            (ERCNKP) isolates was conducted from June 2010 to May 2011 at LMC (314 beds) and from July 2008 to December 2010 at the Tel Aviv Sourasky Medical Center (TASMC) (1,200 beds). Molecular typing was done by arbitrarily primed PCR, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). A total of 8 of 42 (19%) ERCNKP isolates in LMC and 1 of 32 (3.1%) in TASMC belonged to the ST-258 clone. These strains carried the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-2<\/jats:sub>\n            or the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-25<\/jats:sub>\n            extended-spectrum \u03b2-lactamase (ESBL) gene. Sequencing of the\n            <jats:italic>ompK<\/jats:italic>\n            genes showed a frameshift mutation in the\n            <jats:italic>ompK35<\/jats:italic>\n            gene. The fate of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            -carrying plasmid, pKpQIL, was determined by S1 analysis and by PCR of the Tn\n            <jats:italic>4401<\/jats:italic>\n            transposon,\n            <jats:italic>repA<\/jats:italic>\n            , and the truncated\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-9<\/jats:sub>\n            . Plasmid analysis of the ERCNKP ST-258 isolates showed variability in plasmid composition and absence of the Tn\n            <jats:italic>4401<\/jats:italic>\n            transposon and the pKpQIL plasmid. In addition, the ST-258 clone was identified in 35\/35 (100%) of KPC-producing\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            isolates but in none of 62 ertapenem-susceptible\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            isolates collected in the two centers. Our results suggest that ERCNKP ST-258 evolved by loss of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            -carrying plasmid pKpQIL. ERCNKP ST-258 appears to have low epidemic potential.\n          <\/jats:p>","DOI":"10.1128\/jcm.00987-12","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T12:00:20Z","timestamp":1342699220000},"page":"3180-3185","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["A Swordless Knight: Epidemiology and Molecular Characteristics of the\n            <i>bla<\/i>\n            <sub>KPC<\/sub>\n            -Negative Sequence Type 258 Klebsiella pneumoniae Clone"],"prefix":"10.1128","volume":"50","author":[{"given":"Amos","family":"Adler","sequence":"first","affiliation":[{"name":"National Center for Infection Control, Ministry of Health, Tel Aviv, Israel"},{"name":"Division of Epidemiology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Paikin","sequence":"additional","affiliation":[{"name":"Division of Medical Laboratories, Laniado Medical Center, Netanya, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yelena","family":"Sterlin","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Glick","sequence":"additional","affiliation":[{"name":"Division of Medical Laboratories, Laniado Medical Center, Netanya, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rotem","family":"Edgar","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rima","family":"Aronov","sequence":"additional","affiliation":[{"name":"Division of Medical Laboratories, Laniado Medical Center, Netanya, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell J.","family":"Schwaber","sequence":"additional","affiliation":[{"name":"National Center for Infection Control, Ministry of Health, Tel Aviv, Israel"},{"name":"Division of Epidemiology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yehuda","family":"Carmeli","sequence":"additional","affiliation":[{"name":"National Center for Infection Control, Ministry of Health, Tel Aviv, Israel"},{"name":"Division of Epidemiology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02566-10"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr382"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01783-10"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05118-11"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01708236"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/421495"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004982"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01312-09"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06182-11"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4745-4750.2005"},{"key":"e_1_3_2_13_2","volume-title":"Approved standard MS100-S20","author":"Clinical and Laboratory Standards Institute","year":"2011","unstructured":"Clinical and Laboratory Standards Institute. 2011. 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