{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T02:02:26Z","timestamp":1774317746715,"version":"3.50.1"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Chlamydia pecorum<\/jats:named-content>\n            is a significant pathogen of domestic livestock and wildlife. We have developed a\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            -specific multilocus sequence analysis (MLSA) scheme to examine the genetic diversity of and relationships between Australian sheep, cattle, and koala isolates. An MLSA of seven concatenated housekeeping gene fragments was performed using 35 isolates, including 18 livestock isolates (11 Australian sheep, one Australian cow, and six U.S. livestock isolates) and 17 Australian koala isolates. Phylogenetic analyses showed that the koala isolates formed a distinct clade, with limited clustering with\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            isolates from Australian sheep. We identified 11 MLSA sequence types (STs) among Australian\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            isolates, 10 of them novel, with koala and sheep sharing at least one identical ST (designated ST2013Aa). ST23, previously identified in global\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            livestock isolates, was observed here in a subset of Australian bovine and sheep isolates. Most notably, ST23 was found in association with multiple disease states and hosts, providing insights into the transmission of this pathogen between livestock hosts. The complexity of the epidemiology of this disease was further highlighted by the observation that at least two examples of sheep were infected with different\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            STs in the eyes and gastrointestinal tract. We have demonstrated the feasibility of our MLSA scheme for understanding the host relationship that exists between Australian\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            strains and provide the first molecular epidemiological data on infections in Australian livestock hosts.\n          <\/jats:p>","DOI":"10.1128\/jcm.00992-13","type":"journal-article","created":{"date-parts":[[2013,6,6]],"date-time":"2013-06-06T03:54:37Z","timestamp":1370490877000},"page":"2625-2632","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Multilocus Sequence Analysis Provides Insights into Molecular Epidemiology of Chlamydia pecorum Infections in Australian Sheep, Cattle, and Koalas"],"prefix":"10.1128","volume":"51","author":[{"given":"Martina","family":"Jelocnik","sequence":"first","affiliation":[{"name":"Institute for Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca D.","family":"Frentiu","sequence":"additional","affiliation":[{"name":"Institute for Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Timms","sequence":"additional","affiliation":[{"name":"Institute for Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Polkinghorne","sequence":"additional","affiliation":[{"name":"Institute for Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2009075"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/jcpa.2002.0629"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.02.026"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00302-2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(93)90183-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.115.25-26.655"},{"key":"e_1_3_3_8_2","first-page":"437","article-title":"Sporadic bovine encephalomyelitis (Buss disease)","volume":"30","author":"McNutt SH","year":"1940","unstructured":"McNuttSH WallerEF. 1940. Sporadic bovine encephalomyelitis (Buss disease). Cornell Vet. 30:437\u2013448.","journal-title":"Cornell Vet"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1726-1729.2003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063878900100405"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.09.034"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-42-29"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044961"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5664-5672.2004"},{"key":"e_1_3_3_15_2","first-page":"109","volume-title":"Proceedings of the 92nd District Veterinarian's Conference","author":"Watt B","year":"2010","unstructured":"WattB. 2010. Arthritis in sheep, p 109\u2013113. In Proceedings of the 92nd District Veterinarian's Conference. District Veterinarian's Association, Dubbo, New South Wales, Australia."},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.09.003"},{"key":"e_1_3_3_17_2","first-page":"114","volume-title":"Proceedings of the 92nd District Veterinarian's Conference","author":"Pitman B","year":"2010","unstructured":"PitmanB. 2010. A case of sporadic bovine encephalomyelitis (SBE) in Hume LHPA, p 114\u2013115. In Proceedings of the 92nd District Veterinarian's Conference. District Veterinarian's Association, Dubbo, New South Wales, Australia."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1997.tb14176.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5452.443"},{"key":"e_1_3_3_20_2","first-page":"113","article-title":"Infection and disease in reservoir and spillover hosts: determinants of pathogen emergence","volume":"315","author":"Daniels PW","year":"2007","unstructured":"DanielsPW HalpinK HyattA MiddletonD. 2007. Infection and disease in reservoir and spillover hosts: determinants of pathogen emergence. Curr. Top. Microbiol. Immunol. 315:113\u2013131.","journal-title":"Curr. Top. Microbiol. Immunol"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1974.tb05265.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(96)80077-4"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(97)80065-3"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.0054-11"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-77"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2008.06.009"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2214"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-5-53"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3140"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02301-06"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-42"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014179"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.59.030804.121325"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro904"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.03.006"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2010.10.005"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.162832"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.029298-0"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2011.00827.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp187"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2109"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.8.754"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02800.x"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(02)02722-1"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00516.x"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2006.1926"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1071\/MA05065"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-355"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-0233(03)00106-0"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.01.004"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.2.487-502.1993"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1968"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(03)00211-6"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063871102300223"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.7589\/2011-10-298"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00992-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00992-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:46:47Z","timestamp":1646480807000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00992-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":56,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1128\/JCM.00992-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00992-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]},"assertion":[{"value":"2013-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}