{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:06:09Z","timestamp":1788998769071,"version":"build-2803163510"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100018332","name":"Harvard Global Health Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100018332","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Committee of Orebro County"},{"name":"Orebro University Hospital Foundation"},{"DOI":"10.13039\/100001341","name":"Richard and Susan Smith Family Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001341","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            , typically a resident of the oro- or nasopharynx and the causative agent of meningococcal meningitis and meningococcemia, is capable of invading and colonizing the urogenital tract. This can result in urethritis, akin to the syndrome caused by its sister species,\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            , the etiologic agent of gonorrhea. Recently, meningococcal strains associated with outbreaks of urethritis were reported to share genetic characteristics with the gonococcus, raising the question of the extent to which these strains contain features that promote adaptation to the genitourinary niche, making them gonococcus-like and distinguishing them from other\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            strains. Here, we analyzed the genomes of 39 diverse\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            isolates associated with urethritis, collected independently over a decade and across three continents. In particular, we characterized the diversity of the nitrite reductase gene (\n            <jats:italic>aniA<\/jats:italic>\n            ), the factor H-binding protein gene (\n            <jats:italic>fHbp<\/jats:italic>\n            ), and the capsule biosynthetic locus, all of which are loci previously suggested to be associated with urogenital colonization. We observed notable diversity, including frameshift variants, in\n            <jats:italic>aniA<\/jats:italic>\n            and\n            <jats:italic>fHbp<\/jats:italic>\n            and the presence of intact, disrupted, and absent capsule biosynthetic genes, indicating that urogenital colonization and urethritis caused by\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            are possible across a range of meningococcal genotypes. Previously identified allelic patterns in urethritis-associated\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            strains may reflect genetic diversity in the underlying meningococcal population rather than novel adaptation to the urogenital tract.\n          <\/jats:p>","DOI":"10.1128\/jcm.01018-17","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T01:12:32Z","timestamp":1505351552000},"page":"3374-3383","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Genomic Characterization of Urethritis-Associated Neisseria meningitidis Shows that a Wide Range of N. meningitidis Strains Can Cause Urethritis"],"prefix":"10.1128","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4326-2911","authenticated-orcid":false,"given":"Kevin C.","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections, National Reference Laboratory for Sexually Transmitted Infections, Faculty of Medicine and Health, \u00d6rebro University, \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samo","family":"Jeverica","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Kirkcaldy","sequence":"additional","affiliation":[{"name":"Division of STD Prevention, National Center for HIV\/AIDS, Viral Hepatitis, STD and TB Prevention, CDC, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Ohnishi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5646-1314","authenticated-orcid":false,"given":"Yonatan H.","family":"Grad","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T. 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Chan School of Public Health, Boston, Massachusetts, USA"},{"name":"Division of Infectious Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45529-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2302.161434"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2016-052781"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6521a5"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-004-1257-7"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix215"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0154047"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1620971114"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01099-12"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001055"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01101.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01945-06"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1904.111799"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02641.x"},{"key":"e_1_3_3_16_2","first-page":"367","article-title":"Neisseria meningitidis in urogenital infection","volume":"50","author":"Beck A","year":"1974","unstructured":"Beck A Fluker JL Platt DJ . 1974. Neisseria meningitidis in urogenital infection. Br J Vener Dis50:367\u2013369.","journal-title":"Br J Vener Dis"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199110000-00006"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/137.4.458"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw420"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw400"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw288"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2014-15-3-r46"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3304"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.056077-0"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv421"},{"key":"e_1_3_3_28_2","unstructured":"Kwong\nJ\nGon\u00e7alves da Silva\nA\nStinear\nT\nHowden\nB\nSeemann\nT\n. 2017. meningotype: in silico typing for Neisseria meningitidis. https:\/\/github.com\/MDU-PHL\/meningotype."},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts199"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1860-2"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx066"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01018-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01018-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:50:47Z","timestamp":1646473847000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01018-17"}},"subtitle":[],"editor":[{"given":"Andrew B.","family":"Onderdonk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1128\/JCM.01018-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01018-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"2017-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}