{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:34:53Z","timestamp":1787668493304,"version":"build-2736575974"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A multicenter clinical study was conducted to evaluate the performance characteristics of the Abbott RealTi\n            <jats:italic>m<\/jats:italic>\n            e CT\/NG assay, a multiplex real-time PCR assay, for simultaneous detection of\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            and\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            . The specimens were collected from a total of 3,832 male and female subjects at 16 geographically diverse sites. Specimens included male and female urine samples, male urethral swabs, female endocervical swabs, and self-collected and clinician-collected vaginal swabs. Specimens were tested with the automated Abbott RealTi\n            <jats:italic>m<\/jats:italic>\n            e CT\/NG assay, Aptima Combo 2 assay (Gen-Probe), ProbeTec ET CT\/GC assay (Becton Dickinson), and culture for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . The Aptima Combo 2 assay, the ProbeTec assay, and the\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            culture were used as the reference assays. For each subject, a patient infected status (PIS) was determined based on the combined results from the reference assays. The overall prevalence in female subjects was 8.9% for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and 3.8% for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . The overall male prevalence was 18.2% for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and 16.7% for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . The overall sensitivity and specificity of the Abbott RealTi\n            <jats:italic>m<\/jats:italic>\n            e CT\/NG assay were 92.4% and 99.2% for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and 96.9% and 99.7% for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            , respectively. In comparison, the sensitivity and specificity, respectively, for the Aptima Combo 2 assay were 94.5% and 99.0% for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and 96.1% and 99.5% for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            , and those for the ProbeTec ET assay were 90.3% and 99.5% for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and 92.0% and 97.3% for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            in this study. The Abbott RealTi\n            <jats:italic>m<\/jats:italic>\n            e CT\/NG assay offers\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            dual detection with high sensitivity and specificity. The automated assay provides a useful alternative nucleic acid amplification assay for clinical laboratories and clinicians.\n          <\/jats:p>","DOI":"10.1128\/jcm.01019-10","type":"journal-article","created":{"date-parts":[[2010,7,29]],"date-time":"2010-07-29T06:25:34Z","timestamp":1280384734000},"page":"3236-3243","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Performance of the Abbott RealTi\n            <i>m<\/i>\n            e CT\/NG for Detection of\n            <i>Chlamydia trachomatis<\/i>\n            and\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"C. A.","family":"Gaydos","sequence":"first","affiliation":[{"name":"Johns Hopkins University, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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M.","family":"Webb","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Bertuzis","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Miller","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Leckie","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Abravaya","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Guidelines for the laboratory testing of STDs. 2009"},{"key":"e_1_3_2_3_2","unstructured":"Sexually transmitted disease surveillance 2010"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.127-131.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31816d1f6d"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.304-309.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2129-2132.1997"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2133-2135.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.577-584.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3544-3549.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4749-4758.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.OLQ.0000124611.73009.D5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199307000-00004"},{"key":"e_1_3_2_14_2","unstructured":"NCCLS. 2002. 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