{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T20:26:39Z","timestamp":1792355199956,"version":"4.4.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T00:00:00Z","timestamp":1511740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000030","name":"HHS | Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["#U60OE000103"],"award-info":[{"award-number":["#U60OE000103"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-plate formic acid, and ethanol\/formic acid tube extraction methods, were evaluated for their ability to render highly pathogenic organisms nonviable and safe for handling in a biosafety level 2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight-laboratory study to examine the utility of the Bruker Biotyper and Vitek MS MALDI-TOF MS systems and their\n                    <jats:italic>in vitro<\/jats:italic>\n                    diagnostic (IVD), research-use-only, and Security-Relevant databases, as applicable, to accurately identify these agents. Forty-six distinct strains of\n                    <jats:named-content content-type=\"genus-species\">Bacillus anthracis<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Francisella tularensis<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Burkholderia mallei<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Clostridium botulinum<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Brucella suis<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Brucella canis<\/jats:named-content>\n                    were extracted and distributed to participating laboratories for analysis. A total of 35 near-neighbor isolates were also analyzed.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01023-17","type":"journal-article","created":{"date-parts":[[2017,10,11]],"date-time":"2017-10-11T20:40:26Z","timestamp":1507754426000},"page":"3513-3529","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms"],"prefix":"10.1128","volume":"55","author":[{"given":"James T.","family":"Rudrik","sequence":"first","affiliation":[{"name":"Bureau of Laboratories, Michigan Department of Health and Human Services, Lansing, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marty K.","family":"Soehnlen","sequence":"additional","affiliation":[{"name":"Bureau of Laboratories, Michigan Department of Health and Human Services, Lansing, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Perry","sequence":"additional","affiliation":[{"name":"Biodefense Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen M.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Minnesota Department of Health, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanda","family":"Reiter-Kintz","sequence":"additional","affiliation":[{"name":"State Hygienic Laboratory at the University of Iowa, Coralville, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip A.","family":"Lee","sequence":"additional","affiliation":[{"name":"Bureau of Public Health Laboratories, Florida Department of Health, Jacksonville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Pettit","sequence":"additional","affiliation":[{"name":"North Carolina State Laboratory of Public Health, North Carolina Department of Health and Human Services, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Tran","sequence":"additional","affiliation":[{"name":"Bureau of the Public Health Laboratory, New York City Department of Health and Mental Hygiene, New York City, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Swaney","sequence":"additional","affiliation":[{"name":"Texas Department of State Health Services Laboratory, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Report on the potential exposure to anthrax","author":"Centers for Disease Control and Prevention","year":"2014","unstructured":"Centers for Disease Control and Prevention. 11July2014. Report on the potential exposure to anthrax. CDC, Atlanta, GA. https:\/\/www.cdc.gov\/about\/pdf\/lab-safety\/final_anthrax_report.pdf."},{"key":"e_1_3_3_3_2","volume-title":"Report on the potential exposure to Ebola virus","author":"Centers for Disease Control and Prevention","year":"2015","unstructured":"Centers for Disease Control and Prevention. 4February2015. Report on the potential exposure to Ebola virus. CDC, Atlanta, GA. https:\/\/www.cdc.gov\/about\/pdf\/lab-safety\/investigation-into-dec-22-2014-cdc-ebola-event.pdf."},{"key":"e_1_3_3_4_2","volume-title":"Committee for Comprehensive Review of DoD Laboratory Procedures, Processes, and Protocols Associated with Inactivating Bacillus anthracis Spores","author":"Department of Defense","year":"2015","unstructured":"Department of Defense. 13July2015. Review Committee report: inadvertent shipment of live Bacillus anthracis spores by DoD. Committee for Comprehensive Review of DoD Laboratory Procedures, Processes, and Protocols Associated with Inactivating Bacillus anthracis Spores. Department of Defense, Linthicum Heights, MD. https:\/\/www.defense.gov\/Portals\/1\/features\/2015\/0615_lab-stats\/Review-Committee-Report-Final.pdf."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00957-15"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac701822j"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2012.03255.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02709-15"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2013.04.001"},{"key":"e_1_3_3_10_2","volume-title":"Methods for the identification of cultured microorganisms using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry","author":"CLSI","year":"2017","unstructured":"CLSI. 2017. Methods for the identification of cultured microorganisms using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry, 1st ed. CLSI guideline M58. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"1"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2012.11.013"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00813-15"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.10.009"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02680-12"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01803-12"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1563-4"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02420-12"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1415-7"},{"key":"e_1_3_3_19_2","unstructured":"New York City Department of Health and Mental Hygiene. 2015Alert #15: imported brucellosis: recent laboratory exposures requiring prophylaxis and long-term follow-up. https:\/\/a816-health30ssl.nyc.gov\/sites\/nychan\/Lists\/AlertUpdateAdvisoryDocuments\/HAN_Brucella.pdf."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.14043"},{"key":"e_1_3_3_21_2","volume-title":"MALDI Biotyper 3.0 user manual","author":"Bruker Daltonics GmbH","year":"2011","unstructured":"Bruker Daltonics GmbH. 2011. MALDI Biotyper 3.0 user manual. Bruker Daltonics GmbH, Bremen, Germany."},{"key":"e_1_3_3_22_2","first-page":"1338","article-title":"AOAC SMPR 2010.001","volume":"94","author":"AOAC International","year":"2011","unstructured":"AOAC International. 2011. AOAC SMPR 2010.001. Standard method performance requirements for polymerase chain reaction (PCR) methods for detection of Francisella tularensis in aerosol collection filters and\/or liquids. J AOAC Int94:1338\u20131341.","journal-title":"Standard method performance requirements for polymerase chain reaction (PCR) methods for detection of Francisella tularensis in aerosol collection filters and\/or liquids"},{"key":"e_1_3_3_23_2","first-page":"1342","article-title":"AOAC SMPR 2010.002","volume":"94","author":"AOAC International","year":"2011","unstructured":"AOAC International. 2011. AOAC SMPR 2010.002. Standard method performance requirements for polymerase chain reaction (PCR) methods for detection of Yersinia pestis in aerosol collection filters and\/or liquids. J AOAC Int94:1342\u20131346.","journal-title":"Standard method performance requirements for polymerase chain reaction (PCR) methods for detection of Yersinia pestis in aerosol collection filters and\/or liquids"},{"key":"e_1_3_3_24_2","first-page":"1347","article-title":"AOAC SMPR 2010.003","volume":"94","author":"AOAC International","year":"2011","unstructured":"AOAC International. 2011. AOAC SMPR 2010.003. Standard method performance requirements for polymerase chain reaction (PCR) methods for detection of Bacillus anthracis aerosol collection filters and\/or liquids. J AOAC Int94:1347\u20131351.","journal-title":"Standard method performance requirements for polymerase chain reaction (PCR) methods for detection of Bacillus anthracis aerosol collection filters and\/or liquids"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01023-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01023-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T04:51:28Z","timestamp":1646455888000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01023-17"}},"subtitle":[],"editor":[{"given":"Sandra S.","family":"Richter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1128\/JCM.01023-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01023-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"2017-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}