{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:00:02Z","timestamp":1785405602864,"version":"3.56.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"The END (Ending Neglected Diseases) Fund"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2026,3,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Calodium hepaticum<\/jats:italic>\n                      (syn.\n                      <jats:italic toggle=\"yes\">Capillaria hepatica<\/jats:italic>\n                      ) is a zoonotic parasite of murid rodents and other mammals, while also being an important agent of hepatic capillariasis in humans. In a cross-sectional survey of schoolchildren across three provinces (Huambo, U\u00edge, and Zaire) in Angola, we found 39 fecal specimens that were considered positive for\n                      <jats:italic toggle=\"yes\">Trichuris trichiura<\/jats:italic>\n                      eggs using the Kato-Katz thick smear but were negative by quantitative polymerase chain reaction (qPCR). Further morphological examination of these putative\n                      <jats:italic toggle=\"yes\">Trichuris<\/jats:italic>\n                      eggs identified them as having an asymmetrical bipolar plug morphology, resembling capillariid eggs. Molecular characterization through Sanger sequencing and nanopore metabarcoding confirmed these eggs as\n                      <jats:italic toggle=\"yes\">C. hepaticum<\/jats:italic>\n                      , indicating spurious human passage. Once passed in human feces, these eggs may embryonate and become infective in the environment, with the potential to cause hepatic capillariasis in humans. Future research should assess the prevalence of both spurious passage and hepatic capillariasis in humans in Angola, as well as evaluate the impact of misidentification of these nematodes with\n                      <jats:italic toggle=\"yes\">Trichuris<\/jats:italic>\n                      eggs on soil-transmitted helminths surveys.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Calodium hepaticum<\/jats:italic>\n                        causes hepatic capillariasis in humans living in low- to upper-middle income countries. This disease is mainly caused by ingestion of embryonated eggs, whereas consumption of eggs that are unembryonated, such as those found in the livers of infected animals, leads to spurious passage. Notably, unembryonated eggs are morphologically similar to those of\n                        <jats:italic toggle=\"yes\">T. trichiura<\/jats:italic>\n                        , leading to potential misdiagnosis in routine fecal examinations and inaccurate estimates of soil-transmitted helminth prevalence. In this study, we detected\n                        <jats:italic toggle=\"yes\">C. hepaticum<\/jats:italic>\n                        eggs in fecal specimens initially identified as positive for\n                        <jats:italic toggle=\"yes\">T. trichiura<\/jats:italic>\n                        eggs by the Kato-Katz thick smear but tested negative by qPCR. Our findings highlight diagnostic challenges posed by such morphological similarities. Additionally, the detection of\n                        <jats:italic toggle=\"yes\">C. hepaticum<\/jats:italic>\n                        eggs in fecal specimens from school-aged children suggests a potential risk of hepatic capillariasis in this population, underscoring the importance for local health authorities and medical practitioners in Angola to consider this parasite as a potential differential diagnosis in cases of unexplained hepatic dysfunction.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.01026-25","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T13:00:57Z","timestamp":1770642057000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Spurious passage of\n                    <i>Calodium hepaticum<\/i>\n                    in human stools misidentified as\n                    <i>Trichuris<\/i>\n                    spp. infection in school-age children in Angola"],"prefix":"10.1128","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4831-9382","authenticated-orcid":true,"given":"Sze Fui","family":"Hii","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ej9dk98","id-type":"ROR","asserted-by":"publisher"}],"name":"Melbourne Veterinary School, Faculty of Science, The University of Melbourne","place":["Parkville, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4903-1479","authenticated-orcid":true,"given":"Vito","family":"Colella","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ej9dk98","id-type":"ROR","asserted-by":"publisher"}],"name":"Melbourne Veterinary School, Faculty of Science, The University of Melbourne","place":["Parkville, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7532-9316","authenticated-orcid":true,"given":"Adam W.","family":"Bartlett","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03r8z3t63","id-type":"ROR","asserted-by":"publisher"}],"name":"Kirby Institute, University of New South Wales","place":["Sydney, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta S\u00f3lveig","family":"Palmeirim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03r8z3t63","id-type":"ROR","asserted-by":"publisher"}],"name":"Kirby Institute, University of New South Wales","place":["Sydney, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1384-2886","authenticated-orcid":true,"given":"Lucas G.","family":"Huggins","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ej9dk98","id-type":"ROR","asserted-by":"publisher"}],"name":"Melbourne Veterinary School, Faculty of Science, The University of Melbourne","place":["Parkville, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Mendes","sequence":"additional","affiliation":[{"name":"Ministry of Health","place":["Luanda, Angola"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2399-7785","authenticated-orcid":true,"given":"Susana","family":"Vaz Nery","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03r8z3t63","id-type":"ROR","asserted-by":"publisher"}],"name":"Kirby Institute, University of New South Wales","place":["Sydney, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7484-7592","authenticated-orcid":true,"given":"Rebecca","family":"Traub","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ej9dk98","id-type":"ROR","asserted-by":"publisher"}],"name":"Melbourne Veterinary School, Faculty of Science, The University of Melbourne","place":["Parkville, Australia"]},{"id":[{"id":"https:\/\/ror.org\/00r4sry34","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Veterinary Medicine, Murdoch University","place":["Murdoch, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","unstructured":"World Health Organization (WHO). 2024. 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