{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:58:46Z","timestamp":1790056726248,"version":"4.0.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            is the main species responsible for aspergillosis in humans. The diagnosis of aspergillosis remains difficult, and the rapid emergence of azole resistance in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            is worrisome. The aim of this study was to validate the new MycoGENIE\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            real-time PCR kit and to evaluate its performance on clinical samples for the detection of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            and its azole resistance. This multiplex assay detects DNA from the\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            species complex by targeting the multicopy 28S rRNA gene and specific TR\n            <jats:sub>34<\/jats:sub>\n            and L98H mutations in the single-copy-number\n            <jats:italic>cyp51A<\/jats:italic>\n            gene of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . The specificity of\n            <jats:italic>cyp51A<\/jats:italic>\n            mutation detection was assessed by testing DNA samples from 25 wild-type or mutated clinical\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            isolates. Clinical validation was performed on 88 respiratory samples obtained from 62 patients and on 69 serum samples obtained from 16 patients with proven or probable aspergillosis and 13 patients without aspergillosis. The limit of detection was &lt;1 copy for the\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            28S rRNA gene and 6 copies for the\n            <jats:italic>cyp51A<\/jats:italic>\n            gene harboring the TR\n            <jats:sub>34<\/jats:sub>\n            and L98H alterations. No cross-reactivity was detected with various fungi and bacteria. All isolates harboring the TR\n            <jats:sub>34<\/jats:sub>\n            and L98H mutations were accurately detected by quantitative PCR (qPCR) analysis. With respiratory samples, qPCR results showed a sensitivity and specificity of 92.9% and 90.1%, respectively, while with serum samples, the sensitivity and specificity were 100% and 84.6%, respectively. Our study demonstrated that this new real-time PCR kit enables sensitive and rapid detection of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            DNA and azole resistance due to TR\n            <jats:sub>34<\/jats:sub>\n            and L98H mutations in clinical samples.\n          <\/jats:p>","DOI":"10.1128\/jcm.01032-17","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T00:30:27Z","timestamp":1502929827000},"page":"3210-3218","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Molecular Diagnosis of Invasive Aspergillosis and Detection of Azole Resistance by a Newly Commercialized PCR Kit"],"prefix":"10.1128","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2817-3830","authenticated-orcid":false,"given":"Eric","family":"Dannaoui","sequence":"first","affiliation":[{"name":"Universit\u00e9 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