{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:20:06Z","timestamp":1791958806669,"version":"4.3.4"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In this study, we developed rapid and sensitive assays for the detection of\n            <jats:named-content content-type=\"genus-species\">Cladophialophora carrionii<\/jats:named-content>\n            , a common agent of human chromoblastomycosis. The isothermal techniques evaluated were rolling-circle amplification (RCA), multiplex ligation-dependent probe amplification (MLPA), and loop-mediated isothermal amplification (LAMP). The probes for RCA and MLPA were designed with target sequences in the rDNA internal transcribed spacer gene (\n            <jats:italic>ITS<\/jats:italic>\n            ) region, and LAMP primers were designed using the elongation factor 1\u03b1 gene (\n            <jats:italic>EF1<\/jats:italic>\n            ); these probes and primers specifically amplified DNA of isolates of the species. The three techniques were sufficiently specific and sensitive for discriminating target DNA of\n            <jats:named-content content-type=\"genus-species\">C. carrionii<\/jats:named-content>\n            from that of related\n            <jats:named-content content-type=\"genus-species\">Cladophialophora<\/jats:named-content>\n            species and other agents of chromoblastomycosis. RCA, MLPA, and LAMP are advantageous in their reliability and ease of operation compared to standard PCR and conventional methods.\n          <\/jats:p>","DOI":"10.1128\/jcm.01033-14","type":"journal-article","created":{"date-parts":[[2014,7,10]],"date-time":"2014-07-10T02:31:40Z","timestamp":1404959500000},"page":"3531-3535","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Three Isothermal Amplification Techniques for Rapid Identification of Cladophialophora carrionii, an Agent of Human Chromoblastomycosis"],"prefix":"10.1128","volume":"52","author":[{"given":"Shuwen","family":"Deng","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China"},{"name":"Department of Dermatology, First Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China"},{"name":"CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Sybren","family":"de Hoog","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China"},{"name":"CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands"},{"name":"Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, Netherlands"},{"name":"Basic Pathology Department, Federal University of Paran\u00e1 State, Curitiba, Paran\u00e1, Brazil"},{"name":"King Abdulaziz University, Jeddah, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Pan","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. H. G. Gerrits","family":"van den Ende","sequence":"additional","affiliation":[{"name":"CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyue","family":"Yang","sequence":"additional","affiliation":[{"name":"CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiufeng","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institute of Public Health, Guangdong Provincial Centers for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Javad","family":"Najafzadeh","sequence":"additional","affiliation":[{"name":"CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands"},{"name":"Department of Parasitology and Mycology, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqing","family":"Liao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, First Hospital of Peking University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1258\/td.2009.090264"},{"key":"e_1_3_3_3_2","first-page":"235","article-title":"Chromoblastomycosis in Mexico","volume":"396","author":"Lavelle P","year":"1980","unstructured":"LavelleP. 1980. 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A.","family":"Land","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,10]]},"references-count":13,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1128\/JCM.01033-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01033-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10]]},"assertion":[{"value":"2014-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}