{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:49:15Z","timestamp":1791132555791,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            There is a need for accurate diagnosis of intestinal parasite infections in humans, but currently available copromicroscopic techniques have shortcomings, such as low sensitivity. We compared the diagnostic accuracy of a further modified version of the recently developed Flotac technique with that of the widely used formalin-ether concentration technique (FECT) for the detection of intestinal protozoa in human stool samples. Formaldehyde-preserved stool samples from 108 individuals in C\u00f4te d'Ivoire were subjected to the Flotac-400 dual technique, using two different flotation solutions (FS), and to the FECT. Stool samples were examined according to computer-generated random lists by an experienced laboratory technician blinded for the results of each method. Both methods detected the same eight intestinal protozoon species. While the Flotac-400 dual technique (results from both FS combined) found higher prevalences of\n            <jats:named-content content-type=\"genus-species\">Entamoeba coli<\/jats:named-content>\n            (77.8% versus 71.3%,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001),\n            <jats:named-content content-type=\"genus-species\">Blastocystis hominis<\/jats:named-content>\n            (20.4% versus 13.0%,\n            <jats:italic>P<\/jats:italic>\n            = 0.458), and\n            <jats:named-content content-type=\"genus-species\">Giardia intestinalis<\/jats:named-content>\n            (8.3% versus 6.5%,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001), the FECT detected higher prevalences of\n            <jats:named-content content-type=\"genus-species\">Entamoeba histolytica\/Entamoeba dispar<\/jats:named-content>\n            (27.8% versus 20.4%,\n            <jats:italic>P<\/jats:italic>\n            = 0.019) and four species of nonpathogenic intestinal protozoa. The diagnostic agreement between the two methods differed considerably depending on the intestinal protozoon investigated (Cohen's kappa measures; range, 0.01 to 0.46). Our study confirmed that the Flotac-400 dual technique can be utilized for the diagnosis of intestinal protozoon infections in humans. Since Flotac is a sensitive technique for the detection of soil-transmitted helminths and\n            <jats:named-content content-type=\"genus-species\">Schistosoma mansoni<\/jats:named-content>\n            , it might become a viable copromicroscopic technique for the concurrent diagnosis of helminths and intestinal protozoon infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.01035-10","type":"journal-article","created":{"date-parts":[[2011,4,28]],"date-time":"2011-04-28T02:22:14Z","timestamp":1303957334000},"page":"2183-2190","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Comparison of the Flotac-400 Dual Technique and the Formalin-Ether Concentration Technique for Diagnosis of Human Intestinal Protozoon Infection"],"prefix":"10.1128","volume":"49","author":[{"given":"S\u00f6ren L.","family":"Becker","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland"},{"name":"University of Basel, Basel, Switzerland"},{"name":"Center for Infectious Diseases and Travel Medicine, Department of Medicine, University Hospital Freiburg, Freiburg im Breisgau, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent K.","family":"Lohourignon","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Formation et de Recherche (UFR) Biosciences, Universit\u00e9 de Cocody-Abidjan, Abidjan, C\u00f4te d'Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Speich","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland"},{"name":"University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rinaldi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Animal Health, Faculty of Veterinary Medicine, University of Naples Federico II, Regional Center for Monitoring Parasites (CREMOPAR), Regione Campania, Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Knopp","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland"},{"name":"University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli\u00e9zer K.","family":"N'Goran","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Formation et de Recherche (UFR) Biosciences, Universit\u00e9 de Cocody-Abidjan, Abidjan, C\u00f4te d'Ivoire"},{"name":"Centre Suisse de Recherches Scientifiques en C\u00f4te d'Ivoire, Abidjan, C\u00f4te d'Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Cringoli","sequence":"additional","affiliation":[{"name":"Department of Pathology and Animal Health, Faculty of Veterinary Medicine, University of Naples Federico II, Regional Center for Monitoring Parasites (CREMOPAR), Regione Campania, Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrg","family":"Utzinger","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland"},{"name":"University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/bimj.4710370705"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.23.6.545"},{"key":"e_1_3_2_4_2","first-page":"1049","article-title":"Long term formalin-preserved stool specimens for detection of intestinal parasites from school-aged children in Tripoli, Libya","volume":"37","author":"Ben Musa N. 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