{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:01:12Z","timestamp":1792710072065,"version":"4.5.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Clinical echinocandin resistance among\n            <jats:named-content content-type=\"genus-species\">Candida glabrata<\/jats:named-content>\n            strains is increasing, especially in the United States. Antifungal susceptibility testing is considered mandatory to guide therapeutic decisions. However, these methodologies are not routinely performed in the hospital setting due to their complexity and the time needed to obtain reliable results. Echinocandin failure in\n            <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n            is linked exclusively to Fks1p and Fks2p amino acid substitutions, and detection of such substitutions would serve as a surrogate marker to identify resistant isolates. In this work, we report an inexpensive, simple, and quick classical PCR set able to objectively detect the most common mechanisms of echinocandin resistance in\n            <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n            within 4 h. The usefulness of this assay was assessed using a blind collection of 50\n            <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n            strains, including 16\n            <jats:italic>FKS1<\/jats:italic>\n            and\/or\n            <jats:italic>FKS2<\/jats:italic>\n            mutants.\n          <\/jats:p>","DOI":"10.1128\/jcm.01038-14","type":"journal-article","created":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T03:49:51Z","timestamp":1400125791000},"page":"2609-2614","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Set of Classical PCRs for Detection of Mutations in\n            <i>Candida glabrata FKS<\/i>\n            Genes Linked with Echinocandin Resistance"],"prefix":"10.1128","volume":"52","author":[{"given":"Catiana","family":"Dudiuk","sequence":"first","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico 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Litoral, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Jimenez-Ortigosa","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, Rutgers, The State University of New Jersey, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxana G.","family":"Vitale","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET), Santa Fe, Argentina"},{"name":"Unidad de Parasitolog\u00eda, Laboratorio de Micolog\u00eda, Hospital Ramos Mej\u00eda, CONICET, Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Afeltra","sequence":"additional","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Laboratorio de Micolog\u00eda, Hospital Ramos Mej\u00eda, CONICET, Ciudad Aut\u00f3noma de Buenos Aires, Argentina"},{"name":"Catedra 1, 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