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In this multicenter study, we compared the performance of the Simplexa Bordetella Direct kit to those of other molecular assays in detecting and differentiating<jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">B. parapertussis<\/jats:named-content>in nasopharyngeal swab specimens. The limits of detection (LODs) were 150 CFU\/ml or 3 fg\/\u03bcl of DNA for<jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>and 1,500 CFU\/ml or 10 fg\/\u03bcl of DNA for<jats:italic>B. parapertussis<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/jcm.01041-20","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T13:26:48Z","timestamp":1602768408000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Multicenter Performance Evaluation of the Simplexa Bordetella Direct Kit in Nasopharyngeal Swab Specimens"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4178-3307","authenticated-orcid":false,"given":"Siu-Kei","family":"Chow","sequence":"first","affiliation":[{"name":"Department of Microbiology, Laboratories Northwest, MultiCare Health System, Tacoma, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Arbefeville","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Bobby L.","family":"Boyanton","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Beaumont Health, Royal Oak, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily M.","family":"Dault","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Beaumont Health, Royal Oak, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Dunn","sequence":"additional","affiliation":[{"name":"Department of Pathology, Texas Children\u2019s Hospital, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Ferrieri","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wallace","family":"Greene","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Penn State Health Milton S. 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