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Fusion of DNA sequences encoding antigenic proteins is a versatile tool to engineer proteins to be used as sensitizing elements in serological tests. A synthetic gene encoding a chimeric protein containing the C-terminal region of C29 and the N-terminal region of TcP2\u03b2 was constructed. A 236-serum panel, composed of 104 reactive and 132 nonreactive sera to Chagas' disease, was used to evaluate the performance of the chimera. Among the nonreactive sera, 65 were from patients with AID (systemic lupus erythematosus and rheumatoid arthritis) or patients infected with<jats:italic>Leishmania brasiliensis<\/jats:italic>,<jats:italic>Brucella abortus<\/jats:italic>,<jats:italic>Streptococcus pyogenes<\/jats:italic>, or<jats:italic>Toxoplasma gondii<\/jats:italic>. The diagnostic performances of the complete TcP2\u03b2 (TcP2\u03b2<jats:sub>FL<\/jats:sub>) and its N-terminal region (TcP2\u03b2<jats:sub>N<\/jats:sub>) were evaluated. TcP2\u03b2<jats:sub>FL<\/jats:sub>showed unspecific recognition toward leishmaniasis (40%) and AID Abs (58%), while TcP2\u03b2<jats:sub>N<\/jats:sub>showed no unspecific recognition. The diagnostic utility of the chimera was evaluated by analyzing reactivity and comparing the results with those obtained with TcP2\u03b2<jats:sub>N<\/jats:sub>. The chimera reactivity was higher than that of the peptide fractions (0.874 versus 0.564 optical density,<jats:italic>P<\/jats:italic>= 0.0017). The detectability and specificity were both 100% for the whole serum panel tested. We conclude that the obtained chimera shows an improved selectivity and sensitivity compared with other ones previously reported, therefore displaying an optimized performance for<jats:italic>Trypanosoma cruzi<\/jats:italic>infection diagnosis.<\/jats:p>","DOI":"10.1128\/jcm.01043-06","type":"journal-article","created":{"date-parts":[[2006,10,4]],"date-time":"2006-10-04T17:59:53Z","timestamp":1159984793000},"page":"3768-3774","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Design, Construction, and Evaluation of a Specific Chimeric Antigen To Diagnose Chagasic Infection"],"prefix":"10.1128","volume":"44","author":[{"given":"Sebastia\u0301n","family":"Aguirre","sequence":"first","affiliation":[{"name":"Instituto de Tecnologi\u0301a Biolo\u0301gica, INTEBIO, Universidad Nacional del Litoral, Santa Fe, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariel M.","family":"Silber","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Edileuza F.","family":"Brito","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalha\u0303es, Fundac\u0327a\u0303o Oswaldo Cruz, Recife, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u0301a E.","family":"Ribone","sequence":"additional","affiliation":[{"name":"Departamento de Qui\u0301mica Anali\u0301tica, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Rosario, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia M.","family":"Lagier","sequence":"additional","affiliation":[{"name":"Departamento de Qui\u0301mica Anali\u0301tica, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Rosario, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva\u0301n S.","family":"Marcipar","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologi\u0301a Biolo\u0301gica, INTEBIO, Universidad Nacional del Litoral, Santa Fe, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(96)00619-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37897424410.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.53.1.179-185.1986"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1995.tb00197.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.7.2.318-321.2000"},{"key":"e_1_3_2_8_2","first-page":"93","volume":"12","year":"1964","unstructured":"Camargo, E. 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