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In a cross-sectional study (June 2018 to September 2019), we evaluated the diagnostic accuracy of a simple and rapid HIVDR assay (the pan-degenerate amplification and adaptation [PANDAA] assay targeting the mutations K65R, K103NS, M184VI, Y181C, and G190A) compared to Sanger sequencing and next-generation sequencing (NGS).<\/jats:p>","DOI":"10.1128\/jcm.01045-20","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T13:31:46Z","timestamp":1591795906000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Diagnostic Accuracy of Pan-Degenerate Amplification and Adaptation Assay for HIV-1 Drug Resistance Mutation Analysis in Low- and Middle-Income Countries"],"prefix":"10.1128","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3531-7780","authenticated-orcid":false,"given":"Vinie","family":"Kouamou","sequence":"first","affiliation":[{"name":"Department of Medicine, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justen","family":"Manasa","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Katzenstein","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Biomedical Research and Training Institute, Harare, Zimbabwe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan M.","family":"McGregor","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiratidzo E.","family":"Ndhlovu","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tariro","family":"Makadzange","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe"},{"name":"Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30702-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix089"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000001273"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu314"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"Kouamou V Manasa J Katzenstein D McGregor AM Ndhlovu CE Makadzange AT. 2019. 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