{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:37:35Z","timestamp":1790854655928,"version":"4.1.0"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Analysis of borrelia isolates collected from ticks, birds, and rodents from the southeastern United States revealed the presence of well-established populations of\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            sensu stricto,\n            <jats:italic>Borrelia bissettii<\/jats:italic>\n            ,\n            <jats:italic>Borrelia carolinensis<\/jats:italic>\n            , and\n            <jats:italic>Borrelia<\/jats:italic>\n            sp. nov. Multilocus sequence analysis of five genomic loci from seven samples representing\n            <jats:italic>Borrelia<\/jats:italic>\n            sp. nov. isolated from nymphal\n            <jats:italic>Ixodes minor<\/jats:italic>\n            collected in South Carolina showed their close relatedness to California strains known as genomospecies 1 and separation from any other known species of the\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            sensu lato complex. One nucleotide difference in the size of the 5S-23S intergenic spacer region, one substitution in 16S rRNA gene signature nucleotides, and silent nucleotide substitutions in sequences of the gene encoding flagellin and the gene\n            <jats:italic>p66<\/jats:italic>\n            clearly separate\n            <jats:italic>Borrelia<\/jats:italic>\n            sp. nov. isolates from South Carolina into two subgroups. The sequences of isolates of each subgroup share the same restriction fragment length polymorphism patterns of the 5S-23S intergenic spacer region and contain unique signature nucleotides in the 16S rRNA gene. We propose that seven\n            <jats:italic>Borrelia<\/jats:italic>\n            sp. nov. isolates from South Carolina and two California isolates designated as genomospecies 1 comprise a single species, which we name\n            <jats:italic>Borrelia americana<\/jats:italic>\n            sp. nov. The currently recognized geographic distribution of\n            <jats:italic>B. americana<\/jats:italic>\n            is South Carolina and California. All strains are associated with\n            <jats:italic>Ixodes pacificus<\/jats:italic>\n            or\n            <jats:italic>Ixodes minor<\/jats:italic>\n            and their rodent and bird hosts.\n          <\/jats:p>","DOI":"10.1128\/jcm.01050-09","type":"journal-article","created":{"date-parts":[[2009,10,22]],"date-time":"2009-10-22T01:45:18Z","timestamp":1256175918000},"page":"3875-3880","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":99,"title":["Delineation of a New Species of the\n            <i>Borrelia burgdorferi<\/i>\n            Sensu Lato Complex,\n            <i>Borrelia americana<\/i>\n            sp. nov"],"prefix":"10.1128","volume":"47","author":[{"given":"Nataliia","family":"Rudenko","sequence":"first","affiliation":[{"name":"Georgia Southern University, James H. Oliver, Jr. Institute of Arthropodology and Parasitology, Statesboro, Georgia 30460-8056"},{"name":"Biology Centre, Institute of Parasitology AS CR"},{"name":"Faculty of Sciences University of South Bohemia, C\u030ceske\u0301 Bude\u030cjovice 37005, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryna","family":"Golovchenko","sequence":"additional","affiliation":[{"name":"Georgia Southern University, James H. Oliver, Jr. Institute of Arthropodology and Parasitology, Statesboro, Georgia 30460-8056"},{"name":"Biology Centre, Institute of Parasitology AS CR"},{"name":"Faculty of Sciences University of South Bohemia, C\u030ceske\u0301 Bude\u030cjovice 37005, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libor","family":"Grubhoffer","sequence":"additional","affiliation":[{"name":"Biology Centre, Institute of Parasitology AS CR"},{"name":"Faculty of Sciences University of South Bohemia, C\u030ceske\u0301 Bude\u030cjovice 37005, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"James H.","family":"Oliver","sequence":"additional","affiliation":[{"name":"Georgia Southern University, James H. 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