{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T06:17:25Z","timestamp":1766902645441},"reference-count":13,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Currently available serological assays for detection of antibodies to hepatitis C virus (HCV) cannot reliably discriminate acute from chronic HCV infection. We developed a multiplexed, flow-cytometric microsphere immunoassay to measure anti-HCV-IgG reactivities to the core, NS3, NS4, and NS5 HCV recombinant proteins and applied it to 99 serum samples from 24 anti-HCV seroconverters and 141 anti-HCV-IgG and HCV RNA-positive plasma specimens from chronically infected people. Differences in the geometric means or means of signal\/cutoff ratios between the two sample sets were statistically significant for all the antigens tested. A multivariate logistic regression model correctly classified the samples in two groups, with a cross-validation accuracy of 90.8% for the acute group and 97.2% for the chronic group. The immunoassay described has the potential to distinguish acute from chronic HCV infection.<\/jats:p>","DOI":"10.1128\/jcm.01064-10","type":"journal-article","created":{"date-parts":[[2010,11,18]],"date-time":"2010-11-18T03:32:17Z","timestamp":1290051137000},"page":"54-57","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins"],"prefix":"10.1128","volume":"49","author":[{"given":"Aufra C.","family":"Araujo","sequence":"first","affiliation":[{"name":"Laboratory Branch, Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina V.","family":"Astrakhantseva","sequence":"additional","affiliation":[{"name":"Laboratory Branch, Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"RPC Diagnostic Systems, Nizhniy Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard A.","family":"Fields","sequence":"additional","affiliation":[{"name":"Laboratory Branch, Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saleem","family":"Kamili","sequence":"additional","affiliation":[{"name":"Laboratory Branch, Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Chang J. C. et al. 1999. Artificial NS4 mosaic antigen of hepatitis C virus. J. Med. Virol. 59 : 437-450.","DOI":"10.1002\/(SICI)1096-9071(199912)59:4<437::AID-JMV4>3.0.CO;2-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","unstructured":"Chen P. J. et al. 1992. Transient immunoglobulin M antibody response to hepatitis C virus capsid antigen in post transfusion hepatitis C: putative serological marker for acute viral infection. Proc. Natl. Acad. Sci. U. S. A. 89 : 5971-5975.","DOI":"10.1073\/pnas.89.13.5971"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Chung R. T. 2005. Acute hepatitis C virus infection. Clin. Infect. Dis. 41(Suppl. 1) : S14-S17.","DOI":"10.1086\/429490"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","unstructured":"Coppola N. et al. 2009. Improvement in the aetiological diagnosis of acute hepatitis C: a diagnostic protocol based on the anti-HCV-IgM titre and IgG avidity index. J. Clin. 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