{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:18:06Z","timestamp":1791303486820,"version":"4.1.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Infection is an important complication in patients with hematologic malignancies or solid tumors undergoing intensive cytotoxic chemotherapy. In only 20 to 30% of the febrile neutropenic episodes, an infectious agent is detected by conventional cultures. In this prospective study, the performance of broad-range PCR coupled with electrospray ionization time of flight mass spectrometry (PCR\/ESI-MS) technology was compared to conventional blood cultures (BC) in a consecutive series of samples from high-risk hematology patients. In 74 patients, BC and a whole-blood sample for PCR\/ESI-MS (Iridica BAC BSI; Abbott, Carlsbad, CA, USA) were collected at the start of each febrile neutropenic episode and, in case of persistent fever, also at day 5. During 100 different febrile episodes, 105 blood samples were collected and analyzed by PCR\/ESI-MS. There was evidence of a bloodstream infection (BSI) in 36\/105 cases (34%), based on 14 cases with both PCR\/ESI-MS and BC positivity, 17 cases with BC positivity only, and 5 cases with PCR\/ESI-MS positivity only. The sensitivity of PCR\/ESI-MS was 45%, specificity was 93%, and the negative predictive value was 80% compared to blood culture. PCR\/ESI-MS detected definite pathogens (\n            <jats:named-content content-type=\"genus-species\">Fusobacterium nucleatum<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            ) missed by BC, whereas it missed both Gram-negative and Gram-positive organisms detected by BC. PCR\/ESI-MS testing detected additional microorganisms but showed a low sensitivity (45%) compared to BC in neutropenic patients. Our results indicate a lower concordance between BC and PCR\/ESI-MS in the neutropenic population than what has been previously reported in other patient groups with normal white blood cell distribution, and a lower sensitivity than other PCR-based methods.\n          <\/jats:p>","DOI":"10.1128\/jcm.01066-16","type":"journal-article","created":{"date-parts":[[2016,7,21]],"date-time":"2016-07-21T02:53:45Z","timestamp":1469069625000},"page":"2513-2520","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Broad-Range PCR Coupled with Electrospray Ionization Time of Flight Mass Spectrometry for Detection of Bacteremia and Fungemia in Patients with Neutropenic Fever"],"prefix":"10.1128","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4338-1929","authenticated-orcid":false,"given":"S.","family":"Desmet","sequence":"first","affiliation":[{"name":"University Hospitals Leuven, Department of Laboratory Medicine, Leuven, Belgium"},{"name":"KU Leuven, University of Leuven, Department of Microbiology and Immunology, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Maertens","sequence":"additional","affiliation":[{"name":"KU Leuven, University of Leuven, Department of Microbiology and Immunology, Leuven, Belgium"},{"name":"University Hospitals Leuven, Department of Hematology, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Bueselinck","sequence":"additional","affiliation":[{"name":"University Hospitals Leuven, Department of Laboratory Medicine, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Lagrou","sequence":"additional","affiliation":[{"name":"University Hospitals Leuven, Department of Laboratory Medicine, Leuven, Belgium"},{"name":"KU Leuven, University of Leuven, Department of Microbiology and Immunology, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir073"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2007.06.012"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1586\/erm.10.24"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00801-14"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000001249"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140865"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704830"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/588660"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2011.01.003"},{"key":"e_1_3_2_11_2","first-page":"104","article-title":"Molecular approaches in the diagnosis of sepsis in neutropenic patients with haematological malignances","volume":"53","author":"Guido M","year":"2012","unstructured":"Guido M, Quattrocchi M, Zizza A, Pasanisi G, Pavone V, Lobreglio G, Gabutti G, De Donno A. 2012. Molecular approaches in the diagnosis of sepsis in neutropenic patients with haematological malignances. J Prev Med Hyg 53:104\u2013108.","journal-title":"J Prev Med Hyg"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00147-10"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.10.012"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00491-09"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.3.6.643-645.1976"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.15.4.558-561.1982"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/421087"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.02.028"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/MPH.0b013e31829eec78"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2012.10.010"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00567-09"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-014-0385-7"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01066-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01066-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:41:18Z","timestamp":1646473278000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01066-16"}},"subtitle":[],"editor":[{"given":"S. S.","family":"Richter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":21,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1128\/JCM.01066-16"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01066-16","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"2016-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}