{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:40:24Z","timestamp":1791196824441,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Cryptosporidium hominis<\/jats:italic>\n            and\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            are the primary species of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            that infect humans.\n            <jats:italic>C. hominis<\/jats:italic>\n            has an anthroponotic transmission cycle, while\n            <jats:italic>C. parvum<\/jats:italic>\n            is zoonotic, infecting cattle and other ruminants, in addition to humans. Most cryptosporidiosis outbreaks in the United States have been caused by\n            <jats:italic>C. hominis<\/jats:italic>\n            , and this species is often reported as the primary cause of cryptosporidiosis in this country. However, outbreaks account for only 10% of the overall cryptosporidiosis cases, and there are few data on the species that cause sporadic cases. The present study identified the species\/genotypes and subgenotypes of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            in 49 cases of sporadic cryptosporidiosis in Wisconsin during the period from 2003 to 2005. The species\/genotype of isolates was determined by PCR restriction fragment length polymorphism analysis of the 18S rRNA and\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            oocyst wall protein genes. The\n            <jats:italic>C. parvum<\/jats:italic>\n            and\n            <jats:italic>C. hominis<\/jats:italic>\n            isolates were subgenotyped by sequence analysis of the GP60 gene. Forty-four of 49 isolates were identified as\n            <jats:italic>C. parvum<\/jats:italic>\n            , and 1 was identified as\n            <jats:italic>C. hominis<\/jats:italic>\n            . Of the remaining isolates, one was identified as being of the cervine genotype, one was identified as being a cervine genotype variant, and two were identified as being of a novel human genotype, previously reported as W17. Nine different subgenotypes were identified within the\n            <jats:italic>C. parvum<\/jats:italic>\n            species, and two of these were responsible for 60% of the cases. In this study we found that most sporadic cases of cryptosporidiosis in Wisconsin are caused by zoonotic\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species, indicating that zoonotic transmission could be more frequently associated with sporadic cases in the United States.\n          <\/jats:p>","DOI":"10.1128\/jcm.01067-06","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T18:54:05Z","timestamp":1165431245000},"page":"4303-4308","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":192,"title":["Evidence Supporting Zoonotic Transmission of\n            <i>Cryptosporidium<\/i>\n            spp. in Wisconsin"],"prefix":"10.1128","volume":"44","author":[{"given":"Dawn C.","family":"Feltus","sequence":"first","affiliation":[{"name":"North Dakota State University, Fargo, North Dakota 58105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine W.","family":"Giddings","sequence":"additional","affiliation":[{"name":"North Dakota State University, Fargo, North Dakota 58105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brianna L.","family":"Schneck","sequence":"additional","affiliation":[{"name":"North Dakota State University, Fargo, North Dakota 58105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Monson","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Warshauer","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"McEvoy","sequence":"additional","affiliation":[{"name":"North Dakota State University, Fargo, North Dakota 58105"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2744-2747.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2000)086[0838:DFOCPI]2.0.CO;2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0801.010269"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00384-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2000.62.368"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)01630-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0802.010256"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1458-1462.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/10.3201\/eid1006.030325"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3472-3480.2001"},{"key":"e_1_3_2_12_2","first-page":"1","volume":"54","year":"2005","unstructured":"Hlavsa, M. C., J. C. Watson, and M. J. Beach. 2005. Cryptosporidiosis surveillance\u2014United States, 1999-2002. Morb. Mortal. Wkly. Rep.54:1-8.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0901.020265"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4446-4454.2005"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1244"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199407213310304"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.11.3984-3990.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3153-3158.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1180-1183.2000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.995-998.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-199902010-00007"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0803.010194"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0909.030047"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-50-3-293"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0701.010109"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-0834-5"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0304.970423"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.3.1154-1162.2001"},{"key":"e_1_3_2_29_2","first-page":"444","volume":"5","year":"1999","unstructured":"Pieniazek, N. J., F. J. Bornay-Llinares, S. B. Slemenda, A. J. da Silva, I. N. Moura, M. J. Arrowood, O. Ditrich, and D. G. Addiss. 1999. New Cryptosporidium genotypes in HIV-infected persons. Emerg. Infect. Dis.5:444-449.","journal-title":"Emerg. Infect. Dis."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.2944-2951.2004"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.03.020"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00115-8"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4117-4134.2000"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2805-2809.2005"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0404.980424"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2002.00855.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0157-4"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1086\/318090"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5254-5257.2003"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01067-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01067-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T04:20:31Z","timestamp":1646454031000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01067-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12]]},"references-count":39,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1128\/JCM.01067-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01067-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12]]},"assertion":[{"value":"2006-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-09-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}