{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:35:02Z","timestamp":1789014902695,"version":"build-2803163510"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Zygomycetes of the order Mucorales can cause life-threatening infections in humans. These mucormycoses are emerging and associated with a rapid tissue destruction and high mortality. The resistance of Mucorales to antimycotic substances varies between and within clinically important genera such as\n                    <jats:named-content content-type=\"genus-species\">Mucor<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Rhizopus<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Lichtheimia<\/jats:named-content>\n                    . Thus, an accurate diagnosis before onset of antimycotic therapy is recommended. Matrix-assisted laser desorption ionization (MALDI)\u2013time of flight (TOF) mass spectrometry (MS) is a potentially powerful tool to rapidly identify infectious agents on the species level. We investigated the potential of MALDI-TOF MS to differentiate\n                    <jats:named-content content-type=\"genus-species\">Lichtheimia<\/jats:named-content>\n                    species, one of the most important agents of mucormycoses. Using the Bruker Daltonics FlexAnalysis (version 3.0) software package, a spectral database library with\n                    <jats:italic>m\/z<\/jats:italic>\n                    ratios of 2,000 to 20,000 Da was created for 19 type and reference strains of clinically relevant Zygomycetes of the order Mucorales (12 species in 7 genera). The database was tested for accuracy by use of 34 clinical and environmental isolates of\n                    <jats:named-content content-type=\"genus-species\">Lichtheimia<\/jats:named-content>\n                    comprising a total of five species. Our data demonstrate that MALDI-TOF MS can be used to clearly discriminate\n                    <jats:named-content content-type=\"genus-species\">Lichtheimia<\/jats:named-content>\n                    species from other pathogenic species of the Mucorales. Furthermore, the method is suitable to discriminate species within the genus. The reliability and robustness of the MALDI-TOF-based identification are evidenced by high score values (above 2.3) for the designation to a certain species and by moderate score values (below 2.0) for the discrimination between clinically relevant (\n                    <jats:named-content content-type=\"genus-species\">Lichtheimia corymbifera<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">L. ramosa<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">L. ornata<\/jats:named-content>\n                    ) and irrelevant (\n                    <jats:named-content content-type=\"genus-species\">L. hyalospora<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">L. sphaerocystis<\/jats:named-content>\n                    ) species. In total, all 34 strains were unequivocally identified by MALDI-TOF MS with score values of &gt;1.8 down to the generic level, 32 out of 34 of the\n                    <jats:named-content content-type=\"genus-species\">Lichtheimia<\/jats:named-content>\n                    isolates (except CNM-CM 5399 and FSU 10566) were identified accurately with score values of &gt;2 (probable species identification), and 25 of 34 isolates were identified to the species level with score values of &gt;2.3 (highly probable species identification). The MALDI-TOF MS-based method reported here was found to be reproducible and accurate, with low consumable costs and minimal preparation time.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01070-11","type":"journal-article","created":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T08:55:54Z","timestamp":1322816154000},"page":"419-427","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Direct Analysis and Identification of Pathogenic Lichtheimia Species by Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Analyzer-Mediated Mass Spectrometry"],"prefix":"10.1128","volume":"50","author":[{"given":"Wieland","family":"Schr\u00f6dl","sequence":"first","affiliation":[{"name":"University of Leipzig, Faculty of Veterinary Medicine, Institute of Bacteriology and Mycology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilo","family":"Heydel","sequence":"additional","affiliation":[{"name":"University of Leipzig, Faculty of Veterinary Medicine, Institute of Bacteriology and Mycology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker U.","family":"Schwartze","sequence":"additional","affiliation":[{"name":"University of Jena, School of Biology and Pharmacy, Institute of Microbiology, Department of Microbiology and Molecular Biology, Jena, Germany"},{"name":"Leibniz Institute for Natural Product Research and Infection Biology\u2014Hans-Kn\u00f6ll-Institute, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"University of Jena, School of Biology and Pharmacy, Institute of Microbiology, Department of Microbiology and Molecular Biology, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke","family":"Gro\u00dfe-Herrenthey","sequence":"additional","affiliation":[{"name":"University of Leipzig, Faculty of Veterinary Medicine, Institute of Bacteriology and Mycology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grit","family":"Walther","sequence":"additional","affiliation":[{"name":"University of Jena, School of Biology and Pharmacy, Institute of Microbiology, Department of Microbiology and Molecular Biology, Jena, Germany"},{"name":"CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Alastruey-Izquierdo","sequence":"additional","affiliation":[{"name":"Instituto de Salud Carlos III National Centre of Microbiology, Majadahonda (Madrid), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Luis","family":"Rodriguez-Tudela","sequence":"additional","affiliation":[{"name":"Instituto de Salud Carlos III National Centre of Microbiology, Majadahonda (Madrid), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Olias","sequence":"additional","affiliation":[{"name":"Freie Universit\u00e4t Berlin, Department of Veterinary Pathology, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse D.","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Natural Product Research and Infection Biology\u2014Hans-Kn\u00f6ll-Institute, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Sybren","family":"de Hoog","sequence":"additional","affiliation":[{"name":"CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Voigt","sequence":"additional","affiliation":[{"name":"University of Jena, School of Biology and Pharmacy, Institute of Microbiology, Department of Microbiology and Molecular Biology, Jena, Germany"},{"name":"Leibniz Institute for Natural Product Research and Infection Biology\u2014Hans-Kn\u00f6ll-Institute, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03323.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01467-08"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02984.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01270-09"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01744-09"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03398.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02983.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg020"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00403-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02381-10"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362176785190111"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02094-08"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200412000-00003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2008.06.002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3649"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02999.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00389-09"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1637\/9088-092509-Reg.1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01780-09"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.236"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/432579"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/600885"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03456.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00687-09"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02071-09"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.3957-3964.1999"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01070-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01070-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:00:58Z","timestamp":1646460058000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01070-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1128\/JCM.01070-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01070-11","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.716398003.791803058","asserted-by":"object"}]},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2]]},"assertion":[{"value":"2011-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}