{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:35:24Z","timestamp":1790350524726,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    is a bacterial pathogen that can invade the central nervous system (CNS), causing meningoencephalitis and brain abscesses. The diagnosis of CNS listeriosis, based on the isolation of the bacteria in the cerebrospinal fluid (CSF), can be difficult because of previous antibiotic treatment and a low number of bacteria in the CSF. To improve the sensitivity of microbiological diagnosis, we have developed a real-time PCR assay for detecting and quantifying\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    DNA in the CSF. The designed primers specifically amplify the\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    <jats:italic>hly<\/jats:italic>\n                    gene, which encodes listeriolysin O, a pore-forming cytolysin. The PCR assay for the\n                    <jats:italic>hly<\/jats:italic>\n                    gene (PCR-hly) provides reproducible quantitative results over a wide dynamic range of concentrations and was highly sensitive while detecting a single gene copy\/ml. By assaying a large panel of bacterial species, including species secreting pore-forming cytolysin, we determined the specificity of the PCR-hly, which exclusively detects the\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    DNA. We then analyzed 214 CSF samples from patients suspected of having CNS listeriosis. PCR-hly was positive in all cases in which\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    was isolated by culture. Positive PCR-hly of the CSF was also obtained for five additional, clinically confirmed cases of CNS listeriosis for which bacterial cultures were negative presumably due to previous treatment with antibiotics. As a complement to classical bacteriological CSF culture, our designed real-time PCR-hly assay proved to be valuable by enhancing the rapidity and the accuracy of the diagnosis of CNS infection by\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    . In addition, the quantitative results provided may, in some instances, be useful for the follow-up of patients under treatment.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01072-11","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T03:40:31Z","timestamp":1316058031000},"page":"3917-3923","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":93,"title":["Diagnosis of Listeria monocytogenes Meningoencephalitis by Real-Time PCR for the\n                    <i>hly<\/i>\n                    Gene"],"prefix":"10.1128","volume":"49","author":[{"given":"Alban","family":"Le Monnier","sequence":"first","affiliation":[{"name":"D\u00e9partement de Microbiologie, H\u00f4pital Necker-Enfants Malades, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris Descartes, Paris, France"},{"name":"Centre National de R\u00e9f\u00e9rence des Listeria, World Health Organization Collaborating Centre for Foodborne Listeriosis, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Abachin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Microbiologie, H\u00f4pital Necker-Enfants Malades, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Beretti","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Microbiologie, H\u00f4pital Necker-Enfants Malades, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Berche","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Microbiologie, H\u00f4pital Necker-Enfants Malades, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samer","family":"Kayal","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Microbiologie, H\u00f4pital Necker-Enfants Malades, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris Descartes, Paris, France"},{"name":"Present address: D\u00e9partement de Microbiologie, H\u00f4pital Ponchaillou-Universit\u00e9 Rennes 1, Rennes, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1438-4221(00)80039-9"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.485-493.2001"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1998.0218"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90411-W"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.2.568-574.1992"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.5.1066-1067.1988"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-009-0670-3"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.840-862.2004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1553-1558.2001"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1747-1752.2000"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.8.2929-2932.2000"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-67.8.1646"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3819-3822.2004"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.1.165-256.2006"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcped.2008.12.018"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)91765-Z"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.9"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01709483"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.6.1391-1395.1996"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063447"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1405.071395"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365549609037921"},{"key":"e_1_3_3_24_2","first-page":"201","article-title":"Evalution of diagnostic PCR for the detection of Listeria monocytogenes in food products","volume":"43","author":"Gouws P. 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