{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:23:49Z","timestamp":1790137429574,"version":"4.0.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            section\n            <jats:italic>Nigri<\/jats:italic>\n            includes species of interest for animal and human health, although studies on species distribution are limited to human cases. Data on the antifungal susceptibilities and the molecular mechanism of triazole resistance in strains belonging to this section are scant. Forty-two black\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            strains from human patients (16 isolates), animals (14 isolates), and the environment (12 isolates) were molecularly characterized and their\n            <jats:italic>in vitro<\/jats:italic>\n            triazole susceptibilities investigated.\n            <jats:named-content content-type=\"genus-species\">Aspergillus tubingensis<\/jats:named-content>\n            was isolated from humans, animals, and environmental settings, whereas\n            <jats:named-content content-type=\"genus-species\">Aspergillus awamori<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Aspergillus niger<\/jats:named-content>\n            were isolated exclusively from humans. Phylogenetic analyses of \u03b2-tubulin and calmodulin gene sequences were concordant in differentiating\n            <jats:named-content content-type=\"genus-species\">A. tubingensis<\/jats:named-content>\n            from\n            <jats:named-content content-type=\"genus-species\">A. awamori<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">A. niger<\/jats:named-content>\n            . Voriconazole and posaconazole (PSZ) were the most active triazoles. One\n            <jats:named-content content-type=\"genus-species\">A. tubingensis<\/jats:named-content>\n            strain was resistant to itraconazole and PSZ and one\n            <jats:named-content content-type=\"genus-species\">A. niger<\/jats:named-content>\n            strain to PSZ. Sequence analysis of the\n            <jats:italic>cyp51A<\/jats:italic>\n            gene revealed different sequence types within a species, and\n            <jats:named-content content-type=\"genus-species\">A. tubingensis<\/jats:named-content>\n            strains were also phylogenetically distinct from\n            <jats:named-content content-type=\"genus-species\">A. awamori<\/jats:named-content>\n            \/\n            <jats:named-content content-type=\"genus-species\">A. niger<\/jats:named-content>\n            strains according to the strain origin and susceptibility profile. Genetic analysis of the\n            <jats:italic>cyp51A<\/jats:italic>\n            sequences suggests that two nonsynonymous mutations resulting in amino acid substitutions in the CYP51A protein (changes of L to R at position 21 [L21R] and of Q to R at position 228 [Q228R]) might be involved in azole resistance. Though azole resistance in black\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            isolates from animals and rural environments does not represent a threat to public health in Southern Italy, the use of triazoles in the clinical setting needs to better monitored. The\n            <jats:italic>cyp51A<\/jats:italic>\n            sequence is useful for the molecular identification of black\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            , and point mutations in protein sequences could be responsible for azole resistance phenomena.\n          <\/jats:p>","DOI":"10.1128\/jcm.01075-16","type":"journal-article","created":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T06:43:21Z","timestamp":1468478601000},"page":"2365-2372","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Species Distribution and\n            <i>In Vitro<\/i>\n            Azole Susceptibility of Aspergillus Section\n            <i>Nigri<\/i>\n            Isolates from Clinical and Environmental Settings"],"prefix":"10.1128","volume":"54","author":[{"given":"Roberta","family":"Iatta","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Nuccio","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Immediato","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Mosca","sequence":"additional","affiliation":[{"name":"Dipartimento Interdisciplinare di Medicina, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"De Carlo","sequence":"additional","affiliation":[{"name":"Dipartimento Interdisciplinare di Medicina, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Miragliotta","sequence":"additional","affiliation":[{"name":"Dipartimento Interdisciplinare di Medicina, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Parisi","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Apulia and Basilicata, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Crescenzo","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Otranto","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Cafarchia","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:ANTO.0000024907.85688.05"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12394"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv067"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3114\/sim.2007.59.13"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00304-11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myu006"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03233-14"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003633"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00428"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ885"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00585-09"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-012-9529-8"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv045"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.065946-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/588660"},{"key":"e_1_3_2_17_2","volume-title":"Atlas of clinical fungi","author":"de Hoog C","year":"2000","unstructured":"de Hoog C, Guarro J, Gen\u00e9 G, Figueiras M. 2000. 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