{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:29:52Z","timestamp":1761488992026,"version":"3.40.3"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>From August 1999 through July 2002, hyperinvasive<jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>serogroup B (MenB) clonal complexes (CCs), namely, ST-32\/ET-5 (CC32) and ST-41\/44\/lineage 3 (CC41\/44), were predominant in the Western Cape Province of South Africa. This study analyzed MenB invasive isolates from a national laboratory-based surveillance system that were collected from January 2002 through December 2006. Isolates were characterized by pulsed-field gel electrophoresis (PFGE) (<jats:italic>n<\/jats:italic>= 302), and multilocus sequence typing (MLST) and PorA and FetA typing were performed on randomly selected isolates (34\/302, 11%). In total, 2,400 cases were reported, with the highest numbers from Gauteng Province (1,307\/2,400, 54%) and Western Cape Province (393\/2,400, 16%); 67% (1,617\/2,400) had viable isolates and 19% (307\/1,617) were identified as serogroup B. MenB incidence remained stable over time (<jats:italic>P<\/jats:italic>= 0.77) (average incidence, 0.13\/100,000 population [range, 0.10 to 0.16\/100,000 population]). PFGE (302\/307, 98%) divided isolates (206\/302, 68%) into 13 clusters and 96 outliers. The largest cluster, B1, accounted for 25% of isolates (76\/302) over the study period; its prevalence decreased from 43% (20\/47) in 2002 to 13% (8\/62) in 2006 (<jats:italic>P<\/jats:italic>&lt; 0.001), and it was common in the Western Cape (58\/76, 76%). Clusters B2 and B3 accounted for 10% (31\/302) and 6% (19\/302), respectively, and showed no significant change over time and were predominant in Gauteng. Randomly selected isolates from clusters B1, B2, and B3 belonged to CC32, CC41\/44, and the new CC4240\/6688, respectively. Overall, 15 PorA and 12 FetA types were identified. MenB isolates were mostly diverse with no single dominant clone; however, CC32 and CC41\/44 accounted for 35% and the new CC4240\/6688 was the third most prevalent clone.<\/jats:p>","DOI":"10.1128\/jcm.01079-12","type":"journal-article","created":{"date-parts":[[2012,9,13]],"date-time":"2012-09-13T02:32:10Z","timestamp":1347503530000},"page":"3678-3686","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Clonal Analysis of Neisseria meningitidis Serogroup B Strains in South Africa, 2002 to 2006: Emergence of New Clone ST-4240\/6688"],"prefix":"10.1128","volume":"50","author":[{"given":"Chivonne","family":"Moodley","sequence":"first","affiliation":[{"name":"Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa"},{"name":"Medical Research Council, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mignon","family":"du Plessis","sequence":"additional","affiliation":[{"name":"Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa"},{"name":"School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa"},{"name":"Medical Research Council, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kedibone","family":"Ndlangisa","sequence":"additional","affiliation":[{"name":"Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa"},{"name":"School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa"},{"name":"Medical Research Council, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"de Gouveia","sequence":"additional","affiliation":[{"name":"Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa"},{"name":"School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa"},{"name":"Medical Research Council, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith P.","family":"Klugman","sequence":"additional","affiliation":[{"name":"School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa"},{"name":"Medical Research Council, Johannesburg, South Africa"},{"name":"Hubert Department of Global Health, Rollins School of Public Health and Division of Infectious Diseases, School of Medicine, Emory University, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"von Gottberg","sequence":"additional","affiliation":[{"name":"Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa"},{"name":"School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa"},{"name":"Medical Research Council, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"for GERMS-SA (Group for Enteric, Respiratory and Meningeal Disease Surveillance in South Africa)","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_2_2_2","DOI":"10.1016\/0140-6736(91)91961-S"},{"doi-asserted-by":"publisher","key":"e_1_3_2_3_2","DOI":"10.1111\/j.1574-6976.2006.00056.x"},{"doi-asserted-by":"publisher","key":"e_1_3_2_4_2","DOI":"10.1099\/00221287-138-3-523"},{"key":"e_1_3_2_5_2","first-page":"641","article-title":"Multilocus genotypes determined by enzyme electrophoresis of Neisseria meningitidis isolated from patients with systemic disease and from healthy carriers","volume":"132","author":"Caugant DA","year":"1986","unstructured":"CaugantDA . 1986. 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