{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:39:20Z","timestamp":1790692760263,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100007486","name":"Universit\u00e9 Paris Sud","doi-asserted-by":"crossref","award":["EA7361"],"award-info":[{"award-number":["EA7361"]}],"id":[{"id":"10.13039\/501100007486","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-33"],"award-info":[{"award-number":["ANR-10-LABX-33"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-14-JAMR-002"],"award-info":[{"award-number":["ANR-14-JAMR-002"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002738","name":"Assistance Publique - H\u00f4pitaux de Paris","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            As carbapenemase-producing Gram-negative bacilli (CP-GNB) (\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Pseudomonadaceae<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Acinetobacter<\/jats:named-content>\n            spp.) are becoming a major public health issue, there is an urgent need for accurate and fast diagnostic tests. The Amplidiag CarbaR+VRE assay is a multiplex nucleic acid-based\n            <jats:italic>in vitro<\/jats:italic>\n            diagnostic test intended for the detection of CP-GNB and vancomycin-resistant enterococci (VRE) from cultured colonies. We have evaluated its ability to detect carbapenemase genes in 100 well-characterized GNB and in 200 consecutive enterobacterial isolates with reduced susceptibility to carbapenems that were referred to the French National Reference Center for carbapenem resistance. The assay has been validated on purified DNA but also directly on colonies. The Amplidiag CarbaR+VRE assay could detect all KPC, NDM, VIM, IMP, and OXA-48-like variants tested and all acquired carbapenem-hydrolyzing oxacillinases from\n            <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n            (OXA-23, OXA-24\/-40, and OXA-58) as well as the overexpressed chromosomally encoded OXA-51-like \u03b2-lactamase associated with an upstream inserted IS\n            <jats:italic>Aba1<\/jats:italic>\n            . However, as claimed by the manufacturer, other carbapenemases such as GES-like carbapenemases (GES-2, GES-5, and GES-14), GIM-1, AIM-1, SPM-1, DIM-1, OXA-198 in\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            , or OXA-143-like in\n            <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n            were not detected. Amplidiag CarbaR+VRE's performance values were high (100% sensitivity and 99% specificity) as it could detect the five major carbapenemases\u2014NDM, VIM, IMP, KPC, and OXA-48\u2014as well as OXA-type carbapenemases from\n            <jats:named-content content-type=\"genus-species\">Acinetobacter<\/jats:named-content>\n            spp. that are currently emerging also among\n            <jats:named-content content-type=\"genus-species\">Proteus mirabilis<\/jats:named-content>\n            and other enterobacterial isolates. It can provide a result directly from colonies growing on Mueller-Hinton (MH) agar or on selective screening medium in less than 2 h. Further evaluations are now necessary to determine the performance values directly on rectal swabs.\n          <\/jats:p>","DOI":"10.1128\/jcm.01092-17","type":"journal-article","created":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T12:13:24Z","timestamp":1515413604000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of the Amplidiag CarbaR+VRE Kit for Accurate Detection of Carbapenemase-Producing Bacteria"],"prefix":"10.1128","volume":"56","author":[{"given":"Saoussen","family":"Oueslati","sequence":"first","affiliation":[{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Bic\u00eatre Hospital, Le Kremlin-Bic\u00eatre, France"},{"name":"EA7361 \u201cStructure, dynamic, function and expression of broad spectrum \u03b2-lactamases,\u201d Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Girlich","sequence":"additional","affiliation":[{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Bic\u00eatre Hospital, Le Kremlin-Bic\u00eatre, France"},{"name":"EA7361 \u201cStructure, dynamic, function and expression of broad spectrum \u03b2-lactamases,\u201d Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Bic\u00eatre Hospital, Le Kremlin-Bic\u00eatre, France"},{"name":"EA7361 \u201cStructure, dynamic, function and expression of broad spectrum \u03b2-lactamases,\u201d Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"},{"name":"Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur-APHP-Universit\u00e9 Paris-Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9937-9572","authenticated-orcid":false,"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Bic\u00eatre Hospital, Le Kremlin-Bic\u00eatre, France"},{"name":"EA7361 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