{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:58:14Z","timestamp":1790834294552,"version":"4.1.0"},"reference-count":14,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"CHU Saint-Etienne"},{"name":"h\u00f4pital la timone"},{"name":"Facult\u00e9 des sciences et techniques Marrakech"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A study comparing the ICT (immunochromatography technology)\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            IgG and IgM rapid diagnostic test (LDBio Diagnostics, France) with a fully automated system, Architect, was performed on samples from university hospitals of Marseille and Saint-Etienne. A total of 767 prospective sera and 235 selected sera were collected. The panels were selected to test various IgG and IgM parameters. The reference technique,\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            IgGII Western blot analysis (LDBio Diagnostics), was used to confirm the IgG results, and commercial kits Platelia Toxo IgM (Bio-Rad) and Toxo-ISAgA (bioM\u00e9rieux) were used in Saint-Etienne and Marseille, respectively, as the IgM reference techniques. Sensitivity and specificity of the ICT and the Architect IgG assays were compared using a prospective panel. Sensitivity was 100% for the ICT test and 92.1% for Architect (cutoff at 1.6 IU\/ml). The low-IgG-titer serum results confirmed that ICT sensitivity was superior to that of Architect. Specificity was 98.7% (ICT) and 99.8% (Architect IgG). The ICT test is also useful for detecting IgM without IgG and is both sensitive (100%) and specific (100%), as it can distinguish nonspecific IgM from specific\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            IgM. In comparison, IgM sensitivity and specificity on Architect are 96.1% and 99.6%, respectively (cutoff at 0.5 arbitrary units [AU]\/ml). To conclude, this new test overcomes the limitations of automated screening techniques, which are not sensitive enough for IgG and lack specificity for IgM (rare IgM false-positive cases).\n          <\/jats:p>","DOI":"10.1128\/jcm.01106-17","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T00:40:19Z","timestamp":1506559219000},"page":"3395-3404","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Evaluation of a New Immunochromatography Technology Test (LDBio Diagnostics) To Detect Toxoplasma IgG and IgM: Comparison with the Routine Architect Technique"],"prefix":"10.1128","volume":"55","author":[{"given":"Caroline","family":"Mahinc","sequence":"first","affiliation":[{"name":"P\u00f4le de Biologie-Pathologie, Parasitology and Mycology Laboratory, University Hospital of Saint Etienne, Saint Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Flori","sequence":"additional","affiliation":[{"name":"P\u00f4le de Biologie-Pathologie, Parasitology and Mycology Laboratory, University Hospital of Saint Etienne, Saint Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edouard","family":"Delaunay","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, H\u00f4pital de la Timone, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Guillerme","sequence":"additional","affiliation":[{"name":"P\u00f4le de Biologie-Pathologie, Parasitology and Mycology Laboratory, University Hospital of Saint Etienne, Saint Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Charaoui","sequence":"additional","affiliation":[{"name":"P\u00f4le de Biologie-Pathologie, Parasitology and Mycology Laboratory, University Hospital of Saint Etienne, Saint Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Raberin","sequence":"additional","affiliation":[{"name":"P\u00f4le de Biologie-Pathologie, Parasitology and Mycology Laboratory, University Hospital of Saint Etienne, Saint Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamal","family":"Hafid","sequence":"additional","affiliation":[{"name":"P\u00f4le de Biologie-Pathologie, Parasitology and Mycology Laboratory, University Hospital of Saint Etienne, Saint Etienne, France"},{"name":"Unit\u00e9 d'Immunologie et de Physiologie, Facult\u00e9 des Sciences et Techniques, Marrakech, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie","family":"L'Ollivier","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, H\u00f4pital de la Timone, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00011289"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.941-945.2004"},{"key":"e_1_3_2_5_2","first-page":"504","article-title":"Toxoplasma gondii infection in pregnancy: opportunities and pitfalls of serological diagnosis","volume":"12","author":"Sensini A","year":"2006","unstructured":"Sensini A . 2006. Toxoplasma gondii infection in pregnancy: opportunities and pitfalls of serological diagnosis. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis12:504\u2013512.","journal-title":"Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268813002756"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01193-16"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00128-09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2016.10.023"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005670"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.07.013"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.09.009"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00291-7"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.1.174-178.1997"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00182-08"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01106-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01106-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:49:00Z","timestamp":1646473740000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01106-17"}},"subtitle":[],"editor":[{"given":"Michael J.","family":"Loeffelholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":14,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1128\/JCM.01106-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01106-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"2017-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}