{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T06:48:12Z","timestamp":1781592492182,"version":"3.54.5"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100015326","name":"HHS | NIH | OSC | Common Fund","doi-asserted-by":"publisher","award":["R01AI137272"],"award-info":[{"award-number":["R01AI137272"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015326","name":"HHS | NIH | OSC | Common Fund","doi-asserted-by":"publisher","award":["R01AI138978"],"award-info":[{"award-number":["R01AI138978"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2020,11,18]]},"abstract":"<jats:p>\n                    The rise of antimicrobial-resistant pathogens can be attributed to the lack of a rapid pathogen identification (ID) or antimicrobial susceptibility testing (AST), resulting in delayed therapeutic decisions at the point of care. Gonorrhea is usually empirically treated, with no AST results available before treatment, thus contributing to the rapid rise in drug resistance. Here, we present a rapid AST platform using RNA signatures for\n                    <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n                    . Transcriptome sequencing (RNA-seq) followed by bioinformatic tools was applied to explore potential markers in the transcriptome profile of\n                    <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n                    upon minutes of azithromycin exposure.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01152-20","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T10:52:29Z","timestamp":1600944749000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["A Novel Platform Using RNA Signatures To Accelerate Antimicrobial Susceptibility Testing in Neisseria gonorrhoeae"],"prefix":"10.1128","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4402-4410","authenticated-orcid":false,"given":"Marjan M.","family":"Hashemi","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Ram-Mohan","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadya","family":"Andini","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas R.","family":"Gessner","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[{"name":"Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tza-Huei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1123-9036","authenticated-orcid":false,"given":"Samuel","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-anchem-061318-115529"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00607-19"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04320-14"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2011.07.014"},{"key":"e_1_3_4_6_2","first-page":"538","article-title":"Gonorrhea\u2014United States, 1998","volume":"49","author":"Centers for Disease Control and Prevention","year":"2000","unstructured":"Centers for Disease Control and Prevention. 2000. 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