{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:43:32Z","timestamp":1785365012871,"version":"3.55.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Taeniasis due to\n            <jats:italic>Taenia solium<\/jats:italic>\n            is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of\n            <jats:italic>T. solium<\/jats:italic>\n            tapeworm carriers is important to prevent human cysticercosis. Current diagnosis based on microscopic observation of eggs lacks both sensitivity and specificity. In the present study, a nested-PCR assay targeting the\n            <jats:italic>Tso31<\/jats:italic>\n            gene was developed for the specific diagnosis of taeniasis due to\n            <jats:italic>T. solium<\/jats:italic>\n            . Initial specificity and sensitivity testing was performed using stored known\n            <jats:italic>T. solium<\/jats:italic>\n            -positive and -negative samples. The assay was further analyzed under field conditions by conducting a case-control study of pretreatment stool samples collected from a population in an area of endemicity. Using the archived samples, the assay showed 97% (31\/32) sensitivity and 100% (123\/123) specificity. Under field conditions, the assay had 100% sensitivity and specificity using microscopy\/enzyme-linked immunosorbent assay coproantigen testing as the gold standards. The Tso31 nested PCR described here might be a useful tool for the early diagnosis and prevention of taeniasis\/cysticercosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.01172-07","type":"journal-article","created":{"date-parts":[[2007,11,8]],"date-time":"2007-11-08T01:59:47Z","timestamp":1194487187000},"page":"286-289","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Nested PCR for Specific Diagnosis of\n            <i>Taenia solium<\/i>\n            Taeniasis"],"prefix":"10.1128","volume":"46","author":[{"given":"Holger","family":"Mayta","sequence":"first","affiliation":[{"name":"Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland"},{"name":"Department of Microbiology, Facultad de Ciencias y Filosofi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland"},{"name":"Department of Microbiology, Facultad de Ciencias y Filosofi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Prendergast","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janeth P.","family":"Castillo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Facultad de Ciencias y Filosofi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeny O.","family":"Tinoco","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector H.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Facultad de Ciencias y Filosofi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurologicas, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando E.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles R.","family":"Sterling","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0158-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000060686"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(03)00059-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1283-1288.1995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(91)90302-F"},{"key":"e_1_3_2_7_2","first-page":"77","volume":"4","year":"1994","unstructured":"Flisser, A. 1994. 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