{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T18:58:39Z","timestamp":1761418719997},"reference-count":41,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            One of the capabilities developed by bacteria is the ability to gain large fragments of DNA from other bacteria or to lose portions of their own genomes. Among these exchangeable fragments are the genomic islands (GIs). Nine GIs have been identified in\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            , and genomic island 3 (GI-3) is shared by two pathogenic species,\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            . GI-3 encodes mostly unknown proteins. One of the aims of this study was to perform pulsed-field gel electrophoresis (PFGE) on field isolates of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            from Chile to determine whether these isolates are clonally related. Furthermore, we focused on the characterization of GI-3, studying its organization and the genetic conservation of the GI-3 sequence using techniques such as tiling-path PCR (TP-PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR). Our results, after PFGE was performed on 69 field isolates of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            from Chile, showed that the strains were genetically homogeneous. To increase the power of genetic discrimination among these strains, we used multiple locus variable-number tandem-repeat (VNTR) analysis with 16 loci (MLVA-16). The results obtained by MLVA-16 showed that the strains of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            were genetically heterogeneous and that most of them clustered according to their geographic origin. Of the genetic loci studied, panel 2B was the one describing the highest diversity in the analysis, as well as locus Bruce19 in panel 2A. In relation to the study of GI-3, our experimental analysis by TP-PCR identified and confirmed that GI-3 is present in all wild strains of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            , demonstrating the high stability of gene cluster GI-3 in Chilean field strains.\n          <\/jats:p>","DOI":"10.1128\/jcm.01176-10","type":"journal-article","created":{"date-parts":[[2011,5,5]],"date-time":"2011-05-05T01:49:23Z","timestamp":1304560163000},"page":"2461-2469","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of Genomic Island 3 and Genetic Variability of Chilean Field Strains of Brucella abortus"],"prefix":"10.1128","volume":"49","author":[{"given":"Sandra","family":"C\u00e9spedes","sequence":"first","affiliation":[{"name":"Molecular Immunology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepci\u00f3n, Concepci\u00f3n"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"Salgado","sequence":"additional","affiliation":[{"name":"Molecular Immunology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepci\u00f3n, Concepci\u00f3n"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricio","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universidad de Chile, Santiago, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Vidal","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universidad de Chile, Santiago, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel A.","family":"O\u00f1ate","sequence":"additional","affiliation":[{"name":"Molecular Immunology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepci\u00f3n, Concepci\u00f3n"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.12.015"},{"key":"e_1_3_2_3_2","first-page":"25","volume-title":"Brucella: molecular and cellular biology","author":"Bricker B","year":"2004","unstructured":"BrickerB. 2004. 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