{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:41:50Z","timestamp":1791618110275,"version":"4.3.4"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The development and validation of a one-step, single-tube, real-time accelerated reverse-transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the L RNA segment of Rift Valley fever virus (RVFV) are described. The assay was performed at a constant temperature (63\u00b0C), with a real-time follow-up using a LightCycler and a double-stranded-DNA-intercalating fluorochrome. The assay is highly sensitive and comparable to real-time RT-PCR, with a detection limit of \u223c10 RNA copies per assay. However, the RT-LAMP assay is much faster than traditional RT-PCR and generates results in &lt;30 min for most diluted samples. The specificity of the primers was established using other, related arboviruses as well as virus-containing and virus-free sera. The RT-LAMP assay reported here is thus a valuable tool for the rapid detection of RVFV in field diagnostic laboratories.<\/jats:p>","DOI":"10.1128\/jcm.01188-08","type":"journal-article","created":{"date-parts":[[2008,9,17]],"date-time":"2008-09-17T20:55:24Z","timestamp":1221684924000},"page":"3653-3659","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Real-Time Reverse-Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus"],"prefix":"10.1128","volume":"46","author":[{"given":"Christophe N.","family":"Peyrefitte","sequence":"first","affiliation":[{"name":"Unite\u0301 de virologie tropicale, Institut de Me\u0301decine tropicale du Service de Sante\u0301 des Arme\u0301es, BP 46, 13 998 Marseille arme\u0301es, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"Boubis","sequence":"additional","affiliation":[{"name":"Unite\u0301 de virologie tropicale, Institut de Me\u0301decine tropicale du Service de Sante\u0301 des Arme\u0301es, BP 46, 13 998 Marseille arme\u0301es, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Coudrier","sequence":"additional","affiliation":[{"name":"Unite\u0301 de ge\u0301ne\u0301tique mole\u0301culaire des Bunyavirus, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miche\u0300le","family":"Bouloy","sequence":"additional","affiliation":[{"name":"Unite\u0301 de ge\u0301ne\u0301tique mole\u0301culaire des Bunyavirus, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Grandadam","sequence":"additional","affiliation":[{"name":"Unite\u0301 de ge\u0301ne\u0301tique mole\u0301culaire des Bunyavirus, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugues J.","family":"Tolou","sequence":"additional","affiliation":[{"name":"Unite\u0301 de virologie tropicale, Institut de Me\u0301decine tropicale du Service de Sante\u0301 des Arme\u0301es, BP 46, 13 998 Marseille arme\u0301es, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u0301bastien","family":"Plumet","sequence":"additional","affiliation":[{"name":"Unite\u0301 de virologie tropicale, Institut de Me\u0301decine tropicale du Service de Sante\u0301 des Arme\u0301es, BP 46, 13 998 Marseille arme\u0301es, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1210.060596"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02095-06"},{"key":"e_1_3_2_4_2","first-page":"905","volume":"49","year":"2000","unstructured":"Centers for Disease Control and Prevention. 2000. 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