{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:40:20Z","timestamp":1790520020771,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The aim of this study was to ascertain the incidence and clinical significance of metallo-\u03b2-lactamases among\n            <jats:italic>Enterobacter<\/jats:italic>\n            strains isolated from patients with nosocomial infections. We prospectively collected data on patients with\n            <jats:italic>Enterobacter<\/jats:italic>\n            infection during a 13-month period. All of the strains were investigated for antibiotic susceptibility, the presence and expression of metallo-\u03b2-lactamases, and clonality. Of 29 infections (11 involving the urinary tract, 7 pneumonias, 3 skin\/soft tissue infections, 3 intra-abdominal infections, 3 bacteremias, and 2 other infections), 7 (24%) were caused by\n            <jats:italic>Enterobacter cloacae<\/jats:italic>\n            strains harboring a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VIM-1<\/jats:sub>\n            gene associated or not with a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV12<\/jats:sub>\n            gene. Infections caused by VIM-1-producing strains were more frequently associated with a recent prior hospitalization (\n            <jats:italic>P<\/jats:italic>\n            = 0.006), cirrhosis (\n            <jats:italic>P<\/jats:italic>\n            = 0.03), relapse of infection (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001), and more prolonged duration of antibiotic therapy (\n            <jats:italic>P<\/jats:italic>\n            = 0.01) than were other infections. All of the isolates were susceptible to imipenem and meropenem and had\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VIM-1<\/jats:sub>\n            preceded by a weak P1 promoter and inactivated P2 promoters. Most VIM-1-producing\n            <jats:italic>Enterobacter<\/jats:italic>\n            isolates belonged to a main clone, but four different clones were found. Multiclonal VIM-1-producing\n            <jats:italic>E. cloacae<\/jats:italic>\n            infections are difficult to diagnose due to an apparent susceptibility to various beta-lactams, including carbapenems, and are associated with a high relapse rate and a more prolonged duration of antibiotic therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.01193-09","type":"journal-article","created":{"date-parts":[[2009,9,10]],"date-time":"2009-09-10T01:32:15Z","timestamp":1252546335000},"page":"3514-3519","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Infections with VIM-1 Metallo-\u03b2-Lactamase-Producing\n            <i>Enterobacter cloacae<\/i>\n            and Their Correlation with Clinical Outcome"],"prefix":"10.1128","volume":"47","author":[{"given":"Marco","family":"Falcone","sequence":"first","affiliation":[{"name":"Nosocomial Infections Unit, Department of Infectious Diseases, Policlinico Umberto I, University of Rome La Sapienza"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lina","family":"Mezzatesta","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Catania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariagrazia","family":"Perilli","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Technologies, University of L'Aquila"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Forcella","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Technologies, University of L'Aquila"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Giordano","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Policlinico Umberto I, University of Rome La Sapienza, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Cafiso","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Catania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Technologies, University of L'Aquila"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Stefani","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Catania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Venditti","sequence":"additional","affiliation":[{"name":"Nosocomial Infections Unit, Department of Infectious Diseases, Policlinico Umberto I, University of Rome La Sapienza"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm471"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.09.017"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.155"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-008-1040-9"},{"key":"e_1_3_2_6_2","unstructured":"Clin. 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