{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:15:13Z","timestamp":1791231313195,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T00:00:00Z","timestamp":1439856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Whole-genome sequencing (WGS) has emerged today as an ultimate typing tool to characterize\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            outbreaks. However, data analysis and interlaboratory comparability of WGS data are still challenging for most public health laboratories. Therefore, we have developed and evaluated a new\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            typing scheme based on genome-wide gene-by-gene comparisons (core genome multilocus the sequence typing [cgMLST]) to allow for a unique typing nomenclature. Initially, we determined the breadth of the\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            population based on MLST data with a Bayesian approach. Based on the genome sequence data of representative isolates for the whole population, cgMLST target genes were defined and reappraised with 67\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            isolates from two outbreaks and serotype reference strains. The Bayesian population analysis generated five\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            groups. Using all available NCBI RefSeq genomes (\n            <jats:italic>n<\/jats:italic>\n            = 36) and six additionally sequenced strains, all genetic groups were covered. Pairwise comparisons of these 42 genome sequences resulted in 1,701 cgMLST targets present in all 42 genomes with 100% overlap and \u226590% sequence similarity. Overall, \u226599.1% of the cgMLST targets were present in 67 outbreak and serotype reference strains, underlining the representativeness of the cgMLST scheme. Moreover, cgMLST enabled clustering of outbreak isolates with \u226410 alleles difference and unambiguous separation from unrelated outgroup isolates. In conclusion, the novel cgMLST scheme not only improves outbreak investigations but also enables, due to the availability of the automatically curated cgMLST nomenclature, interlaboratory exchange of data that are crucial, especially for rapid responses during transsectorial outbreaks.\n          <\/jats:p>","DOI":"10.1128\/jcm.01193-15","type":"journal-article","created":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T03:40:15Z","timestamp":1435808415000},"page":"2869-2876","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":298,"title":["Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Whole-Genome Sequence-Based Typing of Listeria monocytogenes"],"prefix":"10.1128","volume":"53","author":[{"given":"Werner","family":"Ruppitsch","sequence":"first","affiliation":[{"name":"German-Austrian Binational Consiliary Laboratory for Listeria, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Pietzka","sequence":"additional","affiliation":[{"name":"German-Austrian Binational Consiliary Laboratory for Listeria, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karola","family":"Prior","sequence":"additional","affiliation":[{"name":"Department for Periodontology, University Hospital Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Bletz","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, University Hospital Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haizpea Lasa","family":"Fernandez","sequence":"additional","affiliation":[{"name":"German-Austrian Binational Consiliary Laboratory for Listeria, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Allerberger","sequence":"additional","affiliation":[{"name":"German-Austrian Binational Consiliary Laboratory for Listeria, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dag","family":"Harmsen","sequence":"additional","affiliation":[{"name":"Department for Periodontology, University Hospital Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Mellmann","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, University Hospital Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2015.1003809"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/978-94-017-9457-2_10","volume-title":"Zoonoses\u2014infections affecting humans and animals","author":"Allerberger F","year":"2015","unstructured":"Allerberger F, Bag\u00f3 Z, Huhulescu S, Pietzka A. 2015. Listeriosis: the dark side of refrigeration and ensiling, p 249\u2013286. In Sing A (ed), Zoonoses\u2014infections affecting humans and animals. focus on public health aspects Springer Verlag, Heidelberg, Germany."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-008-1130-2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46495-0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00501-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1574-1585.2003"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12342"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1653-1659.1998"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.2.407-415.1994"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.619-621.2006"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-86"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.757-762.2003"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.2.913-920.2004"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000146"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01930-13"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2012-001124"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022751"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3093"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12638"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3140"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-009-1137-3"},{"issue":"16","key":"e_1_3_3_23_2","first-page":"pii=19477","article-title":"Listeriosis outbreak caused by acid curd cheese Quargel, Austria and Germany 2009","volume":"15","author":"Fretz R","year":"2010","unstructured":"Fretz R, Sagel U, Ruppitsch W, Pietzka A, Stoger A, Huhulescu S, Heuberger S, Pichler J, Much P, Pfaff G, Stark K, Prager R, Flieger A, Feenstra O, Allerberger F. 2010. Listeriosis outbreak caused by acid curd cheese Quargel, Austria and Germany 2009. Euro Surveill 15(16):pii=19477. http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId=19543.","journal-title":"Euro Surveill"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.15.16.19543-en"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.074492.107"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2522"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-539"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003788"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.14.24"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00567-14"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00262-14"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-302"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2010.11.002"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089964"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid\/1811.120453"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030033"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01193-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01193-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:22:05Z","timestamp":1646482925000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01193-15"}},"subtitle":[],"editor":[{"given":"D. J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":36,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1128\/JCM.01193-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01193-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"2015-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-06-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}