{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:52:23Z","timestamp":1792806743752,"version":"4.5.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Toxoplasmosis, a benign infection, is asymptomatic or paucisymptomatic in over 80% of cases, except in immunocompetent patients suffering from ocular toxoplasmosis or in immunocompromised patients with opportunistic or congenital toxoplasmosis. Diagnosis is based mainly on serology testing. Thus, we compared the performance of the nine most commonly used commercial automated or semiautomated immunoassays for IgG and IgM\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            antibody detection, that is, the Advia Centaur, Architect, AxSYM, Elecsys, Enzygnost, Liaison, Platelia, VIDAS, and VIDIA assays. The assays were conducted on four panels of serum samples derived during routine testing from patients with an interfering disease and who exhibited a low IgG antibody level in one of two clinical settings, namely, acute or chronic toxoplasmosis. As a result, IgG sensitivities ranged from 97.1% to 100%, and IgG specificities ranged from 99.5% to 100%. For IgG quantification, strong differences in IgG titers (expressed in IU\/ml) were noted depending on the assay used. IgM sensitivities ranged from 65% to 97.9%, and IgM specificities ranged from 92.6% to 100%. For defining the best serological strategies to be implemented, it appears crucial to compare the diagnostic performance of the different tests with respect to their specificity and sensitivity in detecting the presence of IgG and IgM antibodies.\n          <\/jats:p>","DOI":"10.1128\/jcm.01193-16","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T02:29:35Z","timestamp":1476325775000},"page":"3034-3042","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis"],"prefix":"10.1128","volume":"54","author":[{"given":"O.","family":"Villard","sequence":"first","affiliation":[{"name":"Institut de Parasitologie et de Pathologie Tropicale de Strasbourg, Universit\u00e9 de Strasbourg, H\u00f4pitaux Universitaires de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Cimon","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"L'Ollivier","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, H\u00f4pital de la Timone, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Fricker-Hidalgo","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire A Michallon, Universit\u00e9 Joseph Fourier, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Godineau","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier de Saint-Denis, Saint-Denis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Houze","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, AP-HP, H\u00f4pital Bichat, Universit\u00e9 Paris Descartes, UMR 216, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Paris","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Pelloux","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire A Michallon, Universit\u00e9 Joseph Fourier, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Villena","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, H\u00f4pital Maison Blanche, Centre Hospitalier Universitaire et Universit\u00e9 de Reims Champagne Ardennes, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Candolfi","sequence":"additional","affiliation":[{"name":"Institut de Parasitologie et de Pathologie Tropicale de Strasbourg, Universit\u00e9 de Strasbourg, H\u00f4pitaux Universitaires de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.941-945.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.07.031"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/01443610310001604376"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/154.4.357"},{"key":"e_1_3_3_6_2","first-page":"155","article-title":"Prophylaxis of prenatal toxoplasmosis","volume":"57","author":"Fuente MC","year":"1997","unstructured":"Fuente MC, Bovone NS, Cabral GE. 1997. 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