{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T05:29:37Z","timestamp":1772602177344,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The main aim of our study was to determine the added value of PCR for the diagnosis of Legionnaires\u2019 disease (LD) in routine clinical practice. The specimens were samples submitted for routine diagnosis of pneumonia from December 2002 to November 2005. Patients were evaluated if, in addition to PCR, the results of at least one of the following diagnostic tests were available: (i) culture for\n            <jats:italic>Legionella<\/jats:italic>\n            spp. on buffered charcoal yeast extract agar or (ii) detection of\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            antigen in urine specimens. Of the 151 evaluated patients, 37 (25%) fulfilled the European Working Group on Legionella Infections criteria for a confirmed case of LD (the \u201cgold standard\u201d). An estimated sensitivity, specificity, and overall percent agreement of 86% (32 of 37; 95% confidence interval [CI] = 72 to 95%), 95% (107 of 112; 95% CI = 90 to 98%), and 93% (139 of 149), respectively, were found for 16S rRNA-based PCR, and corresponding values of 92% (34 of 37; 95% CI = 78 to 98%), 98% (110 of 112; 95% CI = 93 to 100%), and 97% (144 of 149), respectively, were found for the\n            <jats:italic>mip<\/jats:italic>\n            gene-based PCR. A total of 35 patients were diagnosed by using the urinary antigen test, and 34 were diagnosed by the 16S rRNA-based PCR. With the\n            <jats:italic>mip<\/jats:italic>\n            gene PCR one more case of LD (\n            <jats:italic>n<\/jats:italic>\n            = 36; not significant) was detected. By combining urinary antigen test and the\n            <jats:italic>mip<\/jats:italic>\n            gene PCR, LD was diagnosed in an additional 4 (11%) patients versus the use of the urinary antigen test alone. The addition of a\n            <jats:italic>L. pneumophila<\/jats:italic>\n            -specific\n            <jats:italic>mip<\/jats:italic>\n            gene PCR to a urinary antigen test is useful in patients with suspected LD who produce sputum and might allow the early detection of a significant number of additional patients.\n          <\/jats:p>","DOI":"10.1128\/jcm.01196-07","type":"journal-article","created":{"date-parts":[[2007,12,20]],"date-time":"2007-12-20T01:45:47Z","timestamp":1198115147000},"page":"671-677","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Utility of Real-Time PCR for Diagnosis of Legionnaires\u2019 Disease in Routine Clinical Practice"],"prefix":"10.1128","volume":"46","author":[{"given":"Bram M. W.","family":"Diederen","sequence":"first","affiliation":[{"name":"Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan A. J. W.","family":"Kluytmans","sequence":"additional","affiliation":[{"name":"Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands"},{"name":"Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina M.","family":"Vandenbroucke-Grauls","sequence":"additional","affiliation":[{"name":"Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel F.","family":"Peeters","sequence":"additional","affiliation":[{"name":"Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.11.4215-4218.2000"},{"key":"e_1_3_2_3_2","first-page":"8","volume":"26","year":"1998","unstructured":"Breiman, F. R., and J. C. Butler. 1998. Legionnaire's disease: clinical, epidemiological, and public health perspectives. Semin. Respir. Infect.26:8-11.","journal-title":"Semin. Respir. Infect."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1709-1712.2000"},{"key":"e_1_3_2_5_2","first-page":"871","volume":"23","year":"2004","unstructured":"den Boer, J. W., and E. P. Yzerman. 2004. Diagnosis of Legionella infection in Legionnaires\u2019 disease. Eur. J. Clin. Microbiol. Infect. Dis.23:871-878.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-1368-9"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46714-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2718-2722.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050427"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/16.6.741"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.506-526.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3247-3254.2005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.375-381.1998"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.40.1.77"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2618-2626.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01695659"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4815-4816.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.4.920-924.1992"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1247-1252.1995"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.12.3325-3328.1993"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.1.199"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.12.3068-3069.1994"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.6.2027-2029.2000"},{"key":"e_1_3_2_24_2","first-page":"79","volume":"38","year":"2002","unstructured":"McNally, C., B. Hackman, B. S. Fields, and J. F. Plouffe. 2002. Potential importance of Legionella species as etiologies in community acquired pneumonia (CAP). Diagn. Microbiol. Infect. Dis.38:79-82.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_25_2","first-page":"54","year":"2006","unstructured":"Mendonca, R., P. Wallace, W. G. NacKay, S. Rottiers, S. Crevecoeur, N. Maes, and M. J. Struelens. 2006. External quality assessment for the detection of Legionella pneumophila by nucleic acid amplification technology (NAAT): a European pilot program, abstr., p. 54. Programme Abstr. 21st Meet. Eur. Working Group Legionella Infect.","journal-title":"External quality assessment for the detection of Legionella pneumophila by nucleic acid amplification technology (NAAT): a European pilot program"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1086\/342884"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/23.3.475"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1264"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.8.2904-2910.2001"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1560-1567.1998"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3814-3817.2002"},{"key":"e_1_3_2_32_2","first-page":"1341","volume":"162","year":"1989","unstructured":"Ruf, B., D. Sh\u00fcrmann, I. Horbach, F. J. Fehrenbach, and H. D. Pohle. 1989. Prevalence and diagnosis of Legionella pneumophila: a 3-year prospective study with emphasis on application of urinary antigen detection. J. Infect. Dis.162:1341-1348.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02839-05"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4016-4021.2003"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.119.1.181"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-1316-8"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.1124-1127.2002"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.9.3985-3993.2001"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3327-3330.2003"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1086\/341087"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3232-3236.2002"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01196-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01196-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:19:45Z","timestamp":1646461185000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01196-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,2]]}},"alternative-id":["10.1128\/JCM.01196-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01196-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2]]},"assertion":[{"value":"2007-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-11-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}