{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:45:07Z","timestamp":1787503507307,"version":"build-2736575974"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100008672","name":"Wilhelm Sander-Stiftung","doi-asserted-by":"publisher","award":["2015.083.1"],"award-info":[{"award-number":["2015.083.1"]}],"id":[{"id":"10.13039\/100008672","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008672","name":"Wilhelm Sander-Stiftung","doi-asserted-by":"publisher","award":["2015.083.1"],"award-info":[{"award-number":["2015.083.1"]}],"id":[{"id":"10.13039\/100008672","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,12,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Invasive fungal infections (IFIs), including aspergillosis and mucormycosis, are serious complications in cancer patients. Reliable detection of etiologic agents is challenging but important for optimal patient management. In a prospective multicenter study, we compared fungal detection by culture, specific and unspecific qPCRs, and fluorescence\n                      <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                      hybridization (FISH) targeting\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      and Mucorales in bronchoalveolar lavage fluid (BALF) and serum of adult cancer patients with suspected fungal pneumonia. In subgroups of patients, fungal DNA was amplified from serum. We evaluated 210 IFI episodes (proven: 3, probable: 72, possible: 107, unclassifiable: 28). Broad-range fungal PCR from BALF was terminated early due to frequent amplification of colonizing yeasts. Specific qPCR assays targeting molds (\n                      <jats:italic toggle=\"yes\">Aspergillus,<\/jats:italic>\n                      Mucorales) were superior for mold detection (26%) compared to FISH (15%) and culture (8%). Detection of fungi by FISH predicted subsequent fungal culture positivity. In proven\/probable IFI episodes, detection of\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      DNA was more likely in BALF than in serum (35\/75 [47%] vs 11\/66 [17%];\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.0002) in contrast to Mucorales DNA (7\/75 [9%] vs 5\/66 [8%];\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.73). Mucorales DNA was detected together with\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      DNA in 6 of 7 BALF samples but not in serum. Specific qPCRs targeting\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      and Mucorales from BALF are the most sensitive means to identify molds in adult cancer patients with suspected fungal pneumonia. BALF qPCRs suggest frequent fungal coinfection, while viruses were rarely detected. Fungal detection by FISH may predict subsequent positive fungal cultures.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>\n                        <jats:bold>IMPORTANCE<\/jats:bold>\n                      <\/jats:title>\n                      <jats:p>\n                        Mold pneumonia is a serious complication in cancer patients. Reliable detection of etiologic agents is critical for patient care, but optimal testing strategies are undefined. We performed a prospective study comparing molecular tests (specific and unspecific qPCR and FISH) and culture on bronchoalveolar lavage fluid (BALF) and serum, focusing on aspergillosis and mucormycosis to gain insights into mold detection in high-risk patients. We demonstrate that FISH visualizes fungal elements in BALF, predominantly conidia, suggesting recent fungal exposure or failure to clear inhaled conidia at the time of diagnostic testing. We find that specific qPCRs from BALF are the most sensitive method to detect\n                        <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                        and Mucorales, superior to broad-range qPCR, culture, and testing of serum. Importantly, Mucorales DNA is mostly co-detected with\n                        <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                        , suggesting co-infections. Although DNA detection in serum is more likely in mucormycosis than in aspergillosis, sole serum testing may miss mucormycosis, precluding optimal patient care.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.01201-25","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T17:48:06Z","timestamp":1764179286000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Molecular diagnostics in cancer patients with suspected respiratory mold infections"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6154-4920","authenticated-orcid":false,"given":"V.","family":"Rickerts","sequence":"first","affiliation":[{"name":"Robert Koch-Institut, Unit 16: Mycotic and Parasitic Agents and Mycobacteria","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Springer","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik II, W\u00fc4i, W\u00fcrzburg University Hospital","place":["W\u00fcrzburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Gerkrath","sequence":"additional","affiliation":[{"name":"Robert Koch-Institut, Unit 16: Mycotic and Parasitic Agents and Mycobacteria","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Korczynski","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00q1fsf04","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hematology, Medical Oncology, and Pneumology, University Medical Center of the Johannes Gutenberg University","place":["Mainz, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Kessel","sequence":"additional","affiliation":[{"name":"Medical Clinic 2, Goethe University Frankfurt","place":["Frankfurt, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Wieters","sequence":"additional","affiliation":[{"name":"Medical Clinic 2, Goethe University Frankfurt","place":["Frankfurt, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01k5qnb77","id-type":"ROR","asserted-by":"publisher"}],"name":"Robert Koch-Institut, Unit 37: Healthcare-Associated Infections, Surveillance of Antibiotic Resistance and Consumption","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Liebregts","sequence":"additional","affiliation":[{"name":"Department of Hematology and Stem Cell Transplantation, University Hospital Essen","place":["Essen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3181-3667","authenticated-orcid":true,"given":"J.","family":"Steinmann","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Excellence Center for Medical Mycology (ECMM), University Hospital Essen","place":["Essen, Germany"]},{"name":"Institute of Clinical Microbiology, Infectious Diseases and Infection Control, Paracelsus Medical University, Klinikum N\u00fcrnberg","place":["N\u00fcrnberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. 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