{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:47:55Z","timestamp":1790696875535,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T00:00:00Z","timestamp":1546387200000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"CIBER de Epidemiologia y Salud Publica"},{"DOI":"10.13039\/501100000805","name":"European Centre for Disease Prevention and Control","doi-asserted-by":"publisher","award":["Pertinent project"],"award-info":[{"award-number":["Pertinent project"]}],"id":[{"id":"10.13039\/501100000805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"MINECO | Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI16\/00247"],"award-info":[{"award-number":["PI16\/00247"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"MINECO | Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI15\/01348"],"award-info":[{"award-number":["PI15\/01348"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:p>\n            This study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification of\n            <jats:named-content content-type=\"genus-species\">Bordetella pertussis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Bordetella parapertussis<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Bordetella holmesii<\/jats:named-content>\n            , as well as its implementation in the diagnostic routine of a reference children\u2019s hospital. The new algorithm included a triplex quantitative PCR (qPCR) targeting IS\n            <jats:italic>481<\/jats:italic>\n            gene (in\n            <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">B. holmesii<\/jats:named-content>\n            , and some\n            <jats:named-content content-type=\"genus-species\">Bordetella bronchiseptica<\/jats:named-content>\n            strains), pIS\n            <jats:italic>1001<\/jats:italic>\n            (\n            <jats:named-content content-type=\"genus-species\">B. parapertussis<\/jats:named-content>\n            -specific) and\n            <jats:italic>rnase<\/jats:italic>\n            P as the human internal control.\n          <\/jats:p>","DOI":"10.1128\/jcm.01231-18","type":"journal-article","created":{"date-parts":[[2018,11,8]],"date-time":"2018-11-08T10:52:44Z","timestamp":1541674364000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain"],"prefix":"10.1128","volume":"57","author":[{"given":"Ana","family":"Valero-Rello","sequence":"first","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desiree","family":"Henares","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Barcelona, Spain"},{"name":"Network of Epidemiology and Public Health, CIBERESP, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesly","family":"Acosta","sequence":"additional","affiliation":[{"name":"Department of Statistics and Operations Research, Universitat Politecnica de Catalunya (BarcelonaTech), Barcelona, Spain"},{"name":"Subdirecci\u00f3 General de Vigil\u00e0ncia i Resposta a Alertas de Salut P\u00fablica (ASPCAT), Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireia","family":"Jane","sequence":"additional","affiliation":[{"name":"Network of Epidemiology and Public Health, CIBERESP, Madrid, Spain"},{"name":"Subdirecci\u00f3 General de Vigil\u00e0ncia i Resposta a Alertas de Salut P\u00fablica (ASPCAT), Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iolanda","family":"Jordan","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Barcelona, Spain"},{"name":"Network of Epidemiology and Public Health, CIBERESP, Madrid, Spain"},{"name":"Pediatric Intensive Care Department, Hospital Sant Joan de D\u00e9u, Universitat de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pere","family":"Godoy","sequence":"additional","affiliation":[{"name":"Network of Epidemiology and Public Health, CIBERESP, Madrid, Spain"},{"name":"Institut de Recerca Biom\u00e8dica de Lleida, Lleida, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Mu\u00f1oz-Almagro","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Barcelona, Spain"},{"name":"Network of Epidemiology and Public Health, CIBERESP, Madrid, Spain"},{"name":"School of Medicine, Universitat Internacional de Catalunya, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.326-382.2005"},{"key":"e_1_3_3_4_2","volume-title":"Epidemiology and prevention of vaccine-preventable diseases","author":"Centers for Disease Control and Prevention","year":"2015","unstructured":"Centers for Disease Control and Prevention. 2015. Epidemiology and prevention of vaccine-preventable diseases, 13th ed. Public Health Foundation, Washington, DC.","edition":"13"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.11.005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088936"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70021-0"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00031-15"},{"key":"e_1_3_3_9_2","first-page":"140","article-title":"Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis","volume":"6","author":"Kolodkina V","year":"2014","unstructured":"Kolodkina V, Martinov V, Babenko A. 2014. Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis. Iran J Microbiol 6:140\u2013148.","journal-title":"Iran J Microbiol"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0195979"},{"key":"e_1_3_3_11_2","volume-title":"Laboratory manual for the diagnosis of whooping cough caused by Bordetella pertussis\/Bordetella parapertussis, update 2014","author":"Guillot S","year":"2014","unstructured":"Guillot S, Guiso N, Riffelmann M, Wirsing Von K\u00f6nig CH. 2014. Laboratory manual for the diagnosis of whooping cough caused by Bordetella pertussis\/Bordetella parapertussis, update 2014. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.009878-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.9.1982-1987.1990"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.1.141-147.1993"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1718-3"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00601-11"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis888"},{"key":"e_1_3_3_18_2","volume-title":"Abstr 27th European Congress of Clinical Microbiology and Infectious Diseases","author":"Valero-Rello A","year":"2017","unstructured":"Valero-Rello A, Henares-Bonilla D, Jordan Garc\u00eda Y, Carmona Parcerisa G, Perez-Arg\u00fcello A, Acosta L, Jan\u00e9 MM-AC. 2017. Validation and implementation of a diagnostic algorithm for the detection of DNA from Bordetella pertussis, B. parapertussis and B. holmesii, based on sequential multiplex and singleplex qPCRs, abstr P0089. Abstr 27th European Congress of Clinical Microbiology and Infectious Diseases, Vienna, Austria, 22 to 25 April 2017."},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2016.1112741"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2016.01.013"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2013.12.003"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00438-14"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2014.03.008"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46331-0"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4121-4126.2003"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.2143\/ACB.3341"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175587"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2311.170960"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01272-11"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06747-11"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ514"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.999-1002.1998"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0b013e3283639c0d"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01231-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01231-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:25:29Z","timestamp":1646475929000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01231-18"}},"subtitle":[],"editor":[{"given":"Daniel J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1128\/JCM.01231-18"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01231-18","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"2018-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01231-18"}}