{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T06:37:36Z","timestamp":1781505456008,"version":"3.54.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2016,9,23]],"date-time":"2016-09-23T00:00:00Z","timestamp":1474588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Macrolide-resistant strains of\n            <jats:named-content content-type=\"genus-species\">Mycoplasma genitalium<\/jats:named-content>\n            are an increasing problem throughout the world, and the implementation of a rapid and sensitive assay for mutation detection to guide treatment is needed. Macrolide-resistant strains have been shown to contain base substitutions in positions 2058 and 2059 (\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            numbering) in region V of the 23S rRNA gene. In this study, we present a SimpleProbe PCR followed by melting curve analysis to differentiate between macrolide-resistant mutants and wild types. The assay was performed on 159\n            <jats:named-content content-type=\"genus-species\">Mycoplasma genitalium<\/jats:named-content>\n            -positive samples, and the results were compared with DNA sequencing. We also looked at the prevalence of macrolide-resistant strains in a Norwegian population. Of 139 samples characterized successfully by sequencing, 54 (39%) were wild types and 85 (61%) were mutants, consisting of 59 (42%) A2059G, 24 (17%) A2058G, 1 (1%) A2058T, and 1 (1%) A2059C mutation. The melting curve analysis correctly differentiated between wild-type and mutant strains in all cases, but it could not identify the different mutant types. The SimpleProbe PCR proved to be a simple, rapid, and reliable method for the detection of macrolide-resistant isolates of\n            <jats:named-content content-type=\"genus-species\">Mycoplasma genitalium<\/jats:named-content>\n            in a clinical setting.\n          <\/jats:p>","DOI":"10.1128\/jcm.01233-16","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T01:58:43Z","timestamp":1470275923000},"page":"2563-2567","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["A Novel SimpleProbe PCR Assay for Detection of Mutations in the 23S rRNA Gene Associated with Macrolide Resistance in Mycoplasma genitalium in Clinical Samples"],"prefix":"10.1128","volume":"54","author":[{"given":"Marianne","family":"Goss\u00e9","sequence":"first","affiliation":[{"name":"Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilde","family":"Lysvand","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brita","family":"Pukstad","sequence":"additional","affiliation":[{"name":"Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway"},{"name":"Department of Dermatology, St. Olavs Hospital HF, Trondheim University Hospital, Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svein Arne","family":"Nordb\u00f8","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Trondheim, Norway"},{"name":"Department of Medical Microbiology, St. Olavs Hospital HF, Trondheim University Hospital, Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-11"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2012.05.006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462413515196"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1043-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr6404a1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir702"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.12.20"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit752"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00495-13"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv136"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/593188"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu217"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu1162"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462413502008"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03318-13"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035593"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12963"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00012-16"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2008.197"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.06.010"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.21734"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.1-12.2001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks263"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00232-10"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000135992.98883.e4"},{"key":"e_1_3_2_27_2","volume-title":"Mykoplasmainfeksjon, genital-veileder for helsepersonell","author":"Blystad H","year":"2015","unstructured":"Blystad H. 2015. Mykoplasmainfeksjon, genital-veileder for helsepersonell. Norwegian Institute of Public Health, Oslo, Norway. http:\/\/www.fhi.no\/artikler\/?id=82826."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.4045\/tidsskr.16.0038"},{"key":"e_1_3_2_29_2","first-page":"455","article-title":"Quinolones: a comprehensive review","volume":"65","author":"Oliphant CM","year":"2002","unstructured":"Oliphant CM, Green GM. 2002. Quinolones: a comprehensive review. 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