{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T01:16:41Z","timestamp":1792891001082,"version":"4.5.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The identification of<jats:italic>Nocardia<\/jats:italic>species, usually based on biochemical tests together with phenotypic<jats:italic>in vitro<\/jats:italic>susceptibility and resistance patterns, is a difficult and lengthy process owing to the slow growth and limited reactivity of these bacteria. In this study, a panel of 153 clinical and reference strains of<jats:italic>Nocardia<\/jats:italic>spp., altogether representing 19 different species, were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). As reference methods for species identification, full-length 16S rRNA gene sequencing and phenotypical biochemical and enzymatic tests were used. In a first step, a complementary homemade reference database was established by the analysis of 110<jats:italic>Nocardia<\/jats:italic>isolates (pretreated with 30 min of boiling and extraction) in the MALDI BioTyper software according to the manufacturer's recommendations for microflex measurement (Bruker Daltonik GmbH, Leipzig, Germany), generating a dendrogram with species-specific cluster patterns. In a second step, the MALDI BioTyper database and the generated database were challenged with 43 blind-coded clinical isolates of<jats:italic>Nocardia<\/jats:italic>spp. Following addition of the homemade database in the BioTyper software, MALDI-TOF MS provided reliable identification to the species level for five species of which more than a single isolate was analyzed. Correct identification was achieved for 38 of the 43 isolates (88%), including 34 strains identified to the species level and 4 strains identified to the genus level according to the manufacturer's log score specifications. These data suggest that MALDI-TOF MS has potential for use as a rapid (&lt;1 h) and reliable method for the identification of<jats:italic>Nocardia<\/jats:italic>species without any substantial costs for consumables.<\/jats:p>","DOI":"10.1128\/jcm.01234-10","type":"journal-article","created":{"date-parts":[[2010,9,23]],"date-time":"2010-09-23T04:40:03Z","timestamp":1285216803000},"page":"4015-4021","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":143,"title":["Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of<i>Nocardia<\/i>Species"],"prefix":"10.1128","volume":"48","author":[{"given":"A.","family":"Verroken","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, UCL Mont-Godinne University Hospital, B-5530 Yvoir"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Janssens","sequence":"additional","affiliation":[{"name":"Microbiology Unit, Faculty of Medicine, Catholic University of Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Berhin","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, UCL Mont-Godinne University Hospital, B-5530 Yvoir"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Bogaerts","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, UCL Mont-Godinne University Hospital, B-5530 Yvoir"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.-D.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, UCL Mont-Godinne University Hospital, B-5530 Yvoir"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Wauters","sequence":"additional","affiliation":[{"name":"Microbiology Unit, Faculty of Medicine, Catholic University of Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Glupczynski","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, UCL Mont-Godinne University Hospital, B-5530 Yvoir"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-009-9193-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.2.259-282.2006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.158-164.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00403-10"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1111\/j.1469-0691.2006.01460.x","volume":"12","year":"2006","unstructured":"Glupczynski, Y., C. Berhin, M. Janssens, and J. Wauters. 2006. Determination of antimicrobial susceptibility patterns of Nocardia spp. from clinical specimens by Etest. Clin. Microbiol. Infect. 12 : 905-912.","journal-title":"from clinical specimens by Etest. Clin. Microbiol. Infect."},{"key":"e_1_3_2_7_2","first-page":"125","volume":"54","year":"2004","unstructured":"Kageyama, A., N. Poonwan, K. Yazawa, Y. Mikami, and K. Nishmura. 2004. Nocardia asiatica sp. nov., isolated from patients with nocardiosis in Japan and clinical specimens from Thailand. Int. J. Syst. Evol. Microbiol. 54 : 125-130.","journal-title":"nov., isolated from patients with nocardiosis in Japan and clinical specimens from Thailand. Int. J. Syst. Evol. Microbiol."},{"key":"e_1_3_2_8_2","first-page":"1927","volume":"54","year":"2004","unstructured":"Kageyama, A., K. Yazawa, A. Mukai, M. Kinoshita, N. Takata, K. Nishmura, R. M. Kroppenstedt, and Y. Mikami. 2004. Nocardia shimofusensis sp. nov., isolated from soil, and Nocardia higoensis sp. nov., isolated from a patient with lung nocardiosis in Japan. Int. J. Syst. Evol. Microbiol. 54 : 1927-1931.","journal-title":"nov., isolated from soil, and"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00157-08"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01959-05"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.851-856.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/600885"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03181.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02071-09"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(88)90025-9"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.017921-0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2624-2628.2005"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01234-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01234-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T00:26:22Z","timestamp":1740529582000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01234-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1128\/JCM.01234-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01234-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11]]},"assertion":[{"value":"2010-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}