{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T17:45:30Z","timestamp":1783532730503,"version":"3.55.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Staphylococcus pseudintermedius<\/jats:named-content>\n            is the most common microorganism isolated from canine pyoderma and postoperative wound infections. The prevalence of methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">S. pseudintermedius<\/jats:named-content>\n            (MRSP) has increased, and recently, isolates that are resistant not only to methicillin but also to other classes of antibiotic drugs, including aminoglycosides, have become common. A total of 422\n            <jats:named-content content-type=\"genus-species\">S. pseudintermedius<\/jats:named-content>\n            isolates collected from 413 dogs were analyzed for amikacin and methicillin resistance using broth microdilution and disk diffusion testing. Methicillin-resistant isolates were significantly (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001) more likely to be resistant to amikacin (37%, 31\/84) than were methicillin-susceptible isolates (7%, 22\/338). Additionally, resistance to non-\u03b2-lactam antibiotics was significantly associated with resistance to amikacin irrespective of methicillin resistance. Among the 422 isolates, 32 that tested positive for amikacin resistance by broth microdilution or disk diffusion testing were investigated further for the presence of aminoglycoside-modifying enzyme genes using multiplex PCR. Of these isolates, 66% (21\/32) were methicillin resistant. In contrast to previous studies of\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            , the most prevalent gene detected was\n            <jats:italic>aph(3<\/jats:italic>\n            \u2032\n            <jats:italic>)-IIIa<\/jats:italic>\n            found in 75% (24\/32) of isolates followed by\n            <jats:italic>aac(6<\/jats:italic>\n            \u2032\n            <jats:italic>)\/aph(2<\/jats:italic>\n            \u2033\n            <jats:italic>)<\/jats:italic>\n            and\n            <jats:italic>ant(4<\/jats:italic>\n            \u2032\n            <jats:italic>)-Ia<\/jats:italic>\n            in 12% (4\/32) and 3% (1\/32), respectively. Understanding the differences in antimicrobial resistance gene carriage between different species of\n            <jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>\n            may improve antimicrobial drug selection for clinical therapy and provide insights into how resistance develops in\n            <jats:named-content content-type=\"genus-species\">S. pseudintermedius<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.01253-14","type":"journal-article","created":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T02:54:29Z","timestamp":1406775269000},"page":"3641-3646","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Amikacin Resistance in Staphylococcus pseudintermedius Isolated from Dogs"],"prefix":"10.1128","volume":"52","author":[{"given":"R. M.","family":"Gold","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University College of Veterinary Medicine and Biomedical Sciences, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. D.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, Texas A&amp;M University College of Veterinary Medicine and Biomedical Sciences, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. D.","family":"Lawhon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University College of Veterinary Medicine and Biomedical Sciences, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2010.00866.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.09.070"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2011.01030.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-009-9313-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.12.018"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.149.3688.1096"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2005.05.002"},{"key":"e_1_3_2_9_2","first-page":"11","article-title":"Mechanisms and epidemiology of aminoglycoside resistance","volume":"36","author":"Brown NM","year":"1992","unstructured":"BrownNMReevesDS. 1992. Mechanisms and epidemiology of aminoglycoside resistance. J. Med. Microbiol. 36:11\u201314.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2003.18.5.631"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks522"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.12.028"},{"key":"e_1_3_2_13_2","volume-title":"Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; second informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2013","unstructured":"Clinical and Laboratory Standards Institute. 2013. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; second informational supplement. CLSI document VET01-S2. 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B.","family":"Onderdonk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,10]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1128\/JCM.01253-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01253-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10]]},"assertion":[{"value":"2014-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}