{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:52:14Z","timestamp":1790671934749,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study evaluated automated and manual commercial DNA extraction methods for their ability to recover DNA from\n            <jats:italic>Brucella<\/jats:italic>\n            species in phosphate-buffered saline (PBS) suspension and from spiked swab specimens. Six extraction methods, representing several of the methodologies which are commercially available for DNA extraction, as well as representing various throughput capacities, were evaluated: the MagNA Pure Compact and the MagNA Pure LC instruments, the IT 1-2-3 DNA sample purification kit, the MasterPure Complete DNA and RNA purification kit, the QIAamp DNA blood mini kit, and the UltraClean microbial DNA isolation kit. These six extraction methods were performed upon three pathogenic\n            <jats:italic>Brucella<\/jats:italic>\n            species:\n            <jats:italic>B. abortus<\/jats:italic>\n            ,\n            <jats:italic>B. melitensis<\/jats:italic>\n            , and\n            <jats:italic>B. suis<\/jats:italic>\n            . Viability testing of the DNA extracts indicated that all six extraction methods were efficient at inactivating virulent\n            <jats:italic>Brucella<\/jats:italic>\n            spp. Real-time PCR analysis using\n            <jats:italic>Brucella<\/jats:italic>\n            genus- and species-specific TaqMan assays revealed that use of the MasterPure kit resulted in superior levels of detection from bacterial suspensions, while the MasterPure kit and MagNA Pure Compact performed equally well for extraction of spiked swab samples. This study demonstrated that DNA extraction methodologies differ in their ability to recover\n            <jats:italic>Brucella<\/jats:italic>\n            DNA from PBS bacterial suspensions and from swab specimens and, thus, that the extraction method used for a given type of sample matrix can influence the sensitivity of real-time PCR assays for\n            <jats:italic>Brucella<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.01288-09","type":"journal-article","created":{"date-parts":[[2009,10,22]],"date-time":"2009-10-22T01:45:18Z","timestamp":1256175918000},"page":"3920-3926","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Evaluation of Automated and Manual Commercial DNA Extraction Methods for Recovery of\n            <i>Brucella<\/i>\n            DNA from Suspensions and Spiked Swabs"],"prefix":"10.1128","volume":"47","author":[{"given":"Leslie A.","family":"Dauphin","sequence":"first","affiliation":[{"name":"Bioterrorism Rapid Response and Advanced Technology (BRRAT) Laboratory, Division of Bioterrorism Preparedness and Response (DBPR), National Center for Preparedness, Detection, and Control of Infectious Diseases (NCPDCID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca J.","family":"Hutchins","sequence":"additional","affiliation":[{"name":"Medical Staffing Network, Inc., 901 Yamato Road, Suite 110, Boca Raton, Florida 33431"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liberty A.","family":"Bost","sequence":"additional","affiliation":[{"name":"Rollins School of Public Health, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Bowen","sequence":"additional","affiliation":[{"name":"Bioterrorism Rapid Response and Advanced Technology (BRRAT) Laboratory, Division of Bioterrorism Preparedness and Response (DBPR), National Center for Preparedness, Detection, and Control of Infectious Diseases (NCPDCID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"387","volume":"50","year":"2004","unstructured":"Al Dahouk, S., H. Tomaso, K. Nockler, and H. Neubauer. 2004. The detection of Brucella spp. using PCR-ELISA and real-time PCR assays. Clin. Lab.50:387-394.","journal-title":"Clin. Lab."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-3-15"},{"key":"e_1_3_2_4_2","unstructured":"Biosafety in microbiological and biomedical laboratories 2007"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.7205\/MILMED.170.4S.39"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.10.009"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.09.004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2762.2009.04404.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-0064-0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01496-07"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3649-3654.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-200206000-00003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.8.2715-2726.2005"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0810.020376"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.07.002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.3012-3016.2002"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3532-3536.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-9"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1252-1255.2003"},{"key":"e_1_3_2_20_2","first-page":"209","volume":"19","year":"1995","unstructured":"Manchester, K. L. 1995. Value of A260\/A280 ratios for measurement of purity of nucleic acis. BioTechniques19:209-210.","journal-title":"BioTechniques"},{"key":"e_1_3_2_21_2","unstructured":"Antisepsis disinfection and sterilization: types action and resistance. 2007"},{"key":"e_1_3_2_22_2","unstructured":"Manual of clinical microbiology 2007"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.8.4753-4759.2003"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050570"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2006.02.002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192319099"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1290-1293.2004"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-007-0409-y"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4211-4217.2002"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1006.030716"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0802.010164"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.09.003"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4616-4622.2005"},{"key":"e_1_3_2_34_2","first-page":"235","volume":"138","year":"1987","unstructured":"Verger, J. M., F. Grimont, P. A. Grimont, and M. Grayon. 1987. Taxonomy of the genus Brucella. Ann. Inst. Pasteur Microbiol.138:235-238.","journal-title":"Ann. Inst. Pasteur Microbiol."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01288-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01288-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:44:01Z","timestamp":1646462641000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01288-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["10.1128\/JCM.01288-09"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01288-09","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12]]},"assertion":[{"value":"2009-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-10-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}